Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.22824
Description:MYB binding protein (P160) 1a
Best-Of-UniGene (BOU) SequenceNM_014520
Genomic Coordiantes Displayed:Bases 216000 to 244000 of contig Hs17_10980_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.22824.jpeg
Best sense EST/protein match:BE888818 matched ref|NP_055335.1| (NM_014520) MYB binding protein (P160) 1a [Homo sapiens] (E = e-105)
Best antisense EST/protein match:BI765222 matched gb|AAH11467.1|AAH11467 (BC011467) Unknown (protein for IMAGE:3154539) [Mus musculus] (E = 2e-77)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BI765222 cDNA clone IMAGE:5190696 pooled colon, kidney, stomach 5' read Protein similarity
  BI758327 cDNA clone IMAGE:5200293 brain 5' read Protein similarity
  BG703494 cDNA clone IMAGE:4818950 hippocampus, cell line 5' read Protein similarity
  BI548663 cDNA clone IMAGE:5276214 hippocampus, cell line 5' read Protein similarity
  H22800 cDNA clone IMAGE:52363 brain 5' read 2.0 kb
  H13309 cDNA clone IMAGE:43360 brain 5' read 1.7 kb
  R19739 cDNA clone IMAGE:34833 brain 5' read 1.6 kb
  BG171161 cDNA clone IMAGE:4427240 hypernephroma, cell line 5' read
  AA359753 cDNA clone ATCC:163872 lung 5' read
  AA339176 cDNA clone ATCC:140895 brain 5' read
  BM142167 cDNA clone (no-name) islets of langerhans 5' read
  BM129745 cDNA clone (no-name) islets of langerhans 5' read
  BG323479 cDNA clone IMAGE:4559910 renal cell adenocarcinoma 5' read
  BI755729 cDNA clone IMAGE:5195505 brain 5' read
  BG698345 cDNA clone IMAGE:4801426 skin 5' read
  F36473 cDNA clone sH5000014-0/D10 muscle
  BE872057 cDNA clone IMAGE:3852058 adenocarcinoma 5' read
  BI489477 cDNA clone IMAGE:5191777 brain 3' read