Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.23765
Description:membrane metallo-endopeptidase-like 2
Best-Of-UniGene (BOU) SequenceNM_033467
Genomic Coordiantes Displayed:Bases 992809 to 1025651 of contig Hs1_4507_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.23765.jpeg
Best sense EST/protein match:NM_033467 matched ref|NP_258428.1| (NM_033467) membrane metallo-endopeptidase-like 2; neprilysin-like (E = 0.0)
Best antisense EST/protein match:BG699669 matched ref|XP_059128.1| (XM_059128) similar to putative (H. sapiens) [Homo sapiens] (E = 1e-88)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI908820 cDNA clone IMAGE:5215316 leukocyte 5' read
  BI908937 cDNA clone IMAGE:5215834 leukocyte 5' read
  BG745636 cDNA clone IMAGE:4850492 spleen 5' read Protein similarity
  BI908668 cDNA clone IMAGE:5215966 leukocyte 5' read Protein similarity
  BI752743 cDNA clone IMAGE:5198655 brain 5' read
  BG699236 cDNA clone IMAGE:4811712 hippocampus, cell line 5' read
  BI551614 cDNA clone IMAGE:5273801 hippocampus, cell line 5' read Protein similarity
  BG699669 cDNA clone IMAGE:4814427 hippocampus, cell line 5' read Protein similarity
  BI459169 cDNA clone IMAGE:5265302 testis, cell line 5' read
  BG700083 cDNA clone IMAGE:4812314 hippocampus, cell line 5' read Protein similarity
  R24961 cDNA clone IMAGE:35078 brain 5' read 2.7 kb
  BI545971 cDNA clone IMAGE:5259575 hippocampus, cell line 5' read
  BF569921 cDNA clone IMAGE:4310080 renal carcinoma (ascites) 5' read
  BE888931 cDNA clone IMAGE:3915206 leiomyosarcoma 5' read
  BM043266 cDNA clone IMAGE:5432929 carcinoma, cell line 5' read
  BF569550 cDNA clone IMAGE:4310341 renal carcinoma (ascites) 5' read
  BM045851 cDNA clone IMAGE:5451418 carcinoma, cell line 5' read
  BG328212 cDNA clone IMAGE:4546827 adenocarcinoma cell line 5' read
  BG387167 cDNA clone IMAGE:4584094 adenocarcinoma cell line 5' read
  BG171694 cDNA clone IMAGE:4425172 hypernephroma, cell line 5' read
  BE513102 cDNA clone IMAGE:3545327 adenocarcinoma cell line 5' read
  R51402 cDNA clone IMAGE:38671 brain 5' read 1.6 kb
  BG422675 cDNA clone IMAGE:4587787 renal cell adenocarcinoma 5' read
  BI161127 cDNA clone IMAGE:5019304 epithelioid carcinoma cell line 5' read
  BE271587 cDNA clone IMAGE:3140801 adenocarcinoma cell line 5' read
  BI824602 cDNA clone IMAGE:5174958 pooled brain, lung, testis 5' read
  BM018328 cDNA clone IMAGE:5427669 astrocytoma grade iv, cell line 5' read
  BM023617 cDNA clone (no-name) islets of langerhans 5' read
  AA326416 cDNA clone ATCC:126947 brain 5' read
  BE548597 cDNA clone IMAGE:3459756 choriocarcinoma 5' read
  AA377857 cDNA clone ATCC:182235 synovial membrane 5' read
  BE879350 cDNA clone IMAGE:3887162 large cell carcinoma, undifferentiated 5' read
  BG772368 cDNA clone IMAGE:4839141 testis, cell line 5' read
  BI858740 cDNA clone IMAGE:5395422 mammary adenocarcinoma, cell line 5' read Polyadenylation signal