Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.241363
Description:hyaluronan-binding protein 2
Best-Of-UniGene (BOU) SequenceNM_004132
Genomic Coordiantes Displayed:Bases 2126000 to 2154000 of contig Hs10_8837_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.241363.jpeg
Best sense EST/protein match:BI761782 matched ref|NP_004123.1| (NM_004132) hyaluronan binding protein 2; hyaluronan-binding (E = e-137)
Best antisense EST/protein match:BF695101 matched dbj|BAB71328.1| (AK056969) unnamed protein product [Homo sapiens] (E = 5e-93)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AJ346143 cDNA clone (no-name) pectoral muscle (after mastectomy) Polyadenylation signal
  F30818 cDNA clone s4000089A05 muscle Polyadenylation signal
  BF475555 cDNA clone IMAGE:3396069 sciatic nerve 3' read Protein similarityPolyadenylation signal
  AA181361 cDNA clone IMAGE:612100 muscle 3' read Protein similarityPolyadenylation signal
  AA085730 cDNA clone IMAGE:562648 muscle 3' read Polyadenylation signal
  F25194 cDNA clone s4000030E06 muscle Polyadenylation signal
  AA086465 cDNA clone IMAGE:562730 muscle 3' read Polyadenylation signal
  F19086 cDNA clone NOTAVAIL03684
  F37524 cDNA clone sH1000004-0/G03 muscle Polyadenylation signal
  F20620 cDNA clone NOTAVAIL04993 muscle
  F20589 cDNA clone NOTAVAIL04960 muscle
  AJ346558 cDNA clone (no-name) pectoral muscle (after mastectomy)
  F20854 cDNA clone 026X4-24 muscle
  F32439 cDNA clone s3000041C07 muscle
  F17933 cDNA clone NOTAVAIL02498
  F21998 cDNA clone 087X1-16 muscle
  F20375 cDNA clone NOTAVAIL04691 muscle
  F29371 cDNA clone s4000076B07 muscle
  F26110 cDNA clone s4000049C01 muscle
  AA193115 cDNA clone IMAGE:628416 muscle 3' read 0.6 kb
  AA180743 cDNA clone IMAGE:612276 muscle 3' read 0.7 kb
  AA180013 cDNA clone IMAGE:611407 muscle 3' read Protein similarity
  F25307 cDNA clone s4000035B03 muscle
  F25153 cDNA clone s4000029E02 muscle
  F37628 cDNA clone NOTAVAIL05399 muscle
  F37937 cDNA clone NOTAVAIL07376 muscle
  F28050 cDNA clone s4000048B09 muscle
  F27533 cDNA clone s4000072A01 muscle
  F31637 cDNA clone s4000123D05 muscle
  F30130 cDNA clone s4000094F02 muscle Protein similarity
  F32146 cDNA clone s3000032B04 muscle Protein similarity
  F31902 cDNA clone s4000131B11 muscle
  AA180799 cDNA clone IMAGE:611407 muscle 5' read Protein similarity
  F18944 cDNA clone NOTAVAIL03541
  F32370 cDNA clone s3000036E12 muscle
  F37738 cDNA clone NOTAVAIL06160 muscle
  F31180 cDNA clone s4000110D06 muscle
  F28506 cDNA clone s3000017F05 muscle Protein similarity
  F26078 cDNA clone s4000048A03 muscle Protein similarity
  C03413 cDNA clone 3NHC1427 heart Protein similarity
  AA128453 cDNA clone IMAGE:562601 muscle 5' read 1.1 kbProtein similarity
  AA181420 cDNA clone IMAGE:612100 muscle 5' read Protein similarity
  AA180913 cDNA clone IMAGE:612276 muscle 5' read 0.7 kbProtein similarity
  C03127 cDNA clone 3NHC0902 heart Protein similarity
  C03126 cDNA clone 3NHC0901 heart Protein similarity
  AA111836 cDNA clone IMAGE:561767 muscle 5' read 1.6 kbProtein similarity
  C02862 cDNA clone 3NHC0439 heart Protein similarity
  AA311751 cDNA clone ATCC:116967 heart 5' read Protein similarity
  C02691 cDNA clone 3NHC0169 heart Protein similarity
  AA195854 cDNA clone IMAGE:628240 muscle 5' read 1.3 kbProtein similarity
  AA192662 cDNA clone IMAGE:628653 muscle 3' read 2.6 kbProtein similarity
  AA176955 cDNA clone IMAGE:611567 muscle 3' read Protein similarity
  AA192963 cDNA clone IMAGE:629601 muscle 3' read Protein similarity
  AA195950 cDNA clone IMAGE:627912 muscle 3' read 1.5 kbProtein similarity
  AA176595 cDNA clone IMAGE:611567 muscle 5' read Protein similarity
  F21243 cDNA clone 046X4-19 muscle Protein similarity
  T30560 cDNA clone ATCC:100365 muscle 5' read Protein similarity
  BF574897 cDNA clone IMAGE:4289643 muscle (skeletal) 5' read Protein similarity
  AA193162 cDNA clone IMAGE:629601 muscle 5' read Protein similarity
  BF670396 cDNA clone IMAGE:4291410 muscle (skeletal) 5' read Protein similarity
  AA196396 cDNA clone IMAGE:627912 muscle 5' read 1.5 kbProtein similarity
  BF695101 cDNA clone IMAGE:4247513 muscle (skeletal) 5' read Protein similarity
  BF671727 cDNA clone IMAGE:4292686 muscle (skeletal) 5' read Protein similarity
  BF694499 cDNA clone IMAGE:4246151 muscle (skeletal) 5' read Protein similarity
  BF670702 cDNA clone IMAGE:4290781 muscle (skeletal) 5' read Protein similarity
  AA194446 cDNA clone IMAGE:628765 muscle 5' read 1.0 kbProtein similarity

SENSE ESTs

  BI761782 cDNA clone IMAGE:5187066 pooled colon, kidney, stomach 5' read Protein similarity
  T50880 cDNA clone IMAGE:78253 liver 5' read 0.4 kbProtein similarity
  BI765113 cDNA clone IMAGE:5191443 pooled colon, kidney, stomach 5' read Protein similarity