Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.245342
Description:hypothetical protein FLJ14642
Best-Of-UniGene (BOU) SequenceNM_032818
Genomic Coordiantes Displayed:Bases 1426000 to 1454000 of contig Hs9_28427_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.245342.jpeg
Best sense EST/protein match:BG826777 matched ref|XP_048479.1| (XM_048479) hypothetical protein FLJ14642 [Homo sapiens] (E = 3e-91)
Best antisense EST/protein match:BE903559 matched gb|AAH18758.1|AAH18758 (BC018758) Similar to RIKEN cDNA 1110032O22 gene [Homo sapiens] (E = 3e-93)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BG105675 cDNA clone IMAGE:4421870 adrenal cortex carcinoma, cell line 5' read
  BI839176 cDNA clone IMAGE:5222634 pooled pancreas and spleen 5' read
  BG424761 cDNA clone IMAGE:4591754 renal cell adenocarcinoma 5' read
  BE903559 cDNA clone IMAGE:3960156 choriocarcinoma 5' read Protein similarity
  BG480211 cDNA clone IMAGE:4653748 choriocarcinoma 5' read
  BG831629 cDNA clone IMAGE:4907976 epithelioid carcinoma cell line 5' read
  BG774335 cDNA clone IMAGE:4810935 choriocarcinoma 5' read
  BI761442 cDNA clone IMAGE:5184446 pooled colon, kidney, stomach 5' read
  W46527 cDNA clone IMAGE:324053 5' read
  W46664 cDNA clone IMAGE:324107 5' read 0.7 kb
  BF675669 cDNA clone IMAGE:4247849 prostate 5' read
  BG546016 cDNA clone IMAGE:4701316 lung 5' read
  AA326790 cDNA clone ATCC:127466 brain 5' read
  T69012 cDNA clone IMAGE:82297 liver 3' read 0.6 kb
  T69093 cDNA clone IMAGE:82297 liver 5' read 0.6 kb
  AI955563 cDNA clone IMAGE:2511650 pancreas 3' read
  W46573 cDNA clone IMAGE:324107 3' read 0.7 kb
  AV761467 cDNA clone MDS3C10 bone marrow 5' read
  AA369471 cDNA clone ATCC:174074 placenta 5' read
  F34322 cDNA clone s3000066G10 muscle
  AA371585 cDNA clone ATCC:176017 brain 5' read
  R43866 cDNA clone IMAGE:33495 brain 3' read 1.0 kb
  AI814718 cDNA clone IMAGE:2420399 pancreas 3' read 0.9 kb
  N83843 cDNA clone KK3663 heart 5' read
  AI870085 cDNA clone IMAGE:2437048 uterus 3' read 1.1 kb


  BG028209 cDNA clone IMAGE:4390447 osteosarcoma, cell line 5' read
  AW673985 cDNA clone IMAGE:2900841 placenta 3' read
  AW872921 cDNA clone IMAGE:3124771 squamous cell carcinoma 3' read
  AA678492 cDNA clone IMAGE:1155871 kidney 3' read Polyadenylation signal
  AI792283 cDNA clone IMAGE:1155871 kidney 5' read 0.5 kb
  BE547681 cDNA clone IMAGE:3462233 choriocarcinoma 5' read
  BE535435 cDNA clone IMAGE:3445342 placenta 5' read
  BE728707 cDNA clone IMAGE:3833669 melanotic melanoma 5' read
  BG333790 cDNA clone IMAGE:4577425 melanotic melanoma 5' read
  BE383670 cDNA clone IMAGE:3614198 melanotic melanoma 5' read
  BF027513 cDNA clone IMAGE:3954847 melanotic melanoma 5' read
  BF026890 cDNA clone IMAGE:3953864 melanotic melanoma 5' read
  BF026886 cDNA clone IMAGE:3953862 melanotic melanoma 5' read
  BE279169 cDNA clone IMAGE:3140025 choriocarcinoma 5' read
  AW674851 cDNA clone IMAGE:2900841 placenta 5' read
  BE735072 cDNA clone IMAGE:3844116 choriocarcinoma 5' read
  BE734711 cDNA clone IMAGE:3842504 choriocarcinoma 5' read
  AI721204 cDNA clone IMAGE:2333836 colon 3' read
  AU123577 cDNA clone NT2RM2000569 5' read
  BE782092 cDNA clone IMAGE:3871338 retinoblastoma 5' read
  BG826777 cDNA clone IMAGE:4904096 rhabdomyosarcoma 5' read Protein similarity
  BI091376 cDNA clone IMAGE:5000577 placenta 5' read
  BI088661 cDNA clone IMAGE:4992870 placenta 5' read
  BE784944 cDNA clone IMAGE:3881803 large cell carcinoma 5' read
  BE018581 cDNA clone IMAGE:3048886 placenta 5' read