Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.247118
Description:phosphatidylinositol glycan, class B
Best-Of-UniGene (BOU) SequenceNM_004855
Genomic Coordiantes Displayed:Bases 4080000 to 4220000 of contig Hs15_10446_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.247118.jpeg
Best sense EST/protein match:AU117795 matched ref|NP_004846.1| (NM_004855) phosphatidylinositol glycan, class B [Homo sapiens] (E = e-153)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA385706 cDNA clone ATCC:189881 thyroid 5' read
  BF964600 cDNA clone IMAGE:4356147 muscle (skeletal) 5' read Protein similarity
  AW769443 cDNA clone IMAGE:3005932 2 pooled wilms' tumors, one primary and one metastatic to 3' read Protein similarityPolyadenylation signal
  AI371418 cDNA clone IMAGE:1915221 kidney 3' read 1.4 kbProtein similarity
  D79614 cDNA clone GEN289G03 aorta 5' read Protein similarity
  AU144243 cDNA clone HEMBA1001328 whole embryo, mainly head 3' read Protein similarity
  AA369939 cDNA clone ATCC:174490 prostate 3' read Protein similarityPolyadenylation signal
  AA740595 cDNA clone IMAGE:1325345 kidney 3' read 0.8 kbProtein similarityPolyadenylation signal
  BI114741 cDNA clone IMAGE:5020915 rhabdomyosarcoma 5' read Protein similarity
  BG282207 cDNA clone IMAGE:4545614 melanotic melanoma 5' read
  AV701234 cDNA clone ADAAGC11 adrenal gland 5' read Protein similarity
  BF311985 cDNA clone IMAGE:4127264 neuroblastoma 5' read Protein similarity
  BM083547 cDNA clone IMAGE:4654489 embryonal carcinoma 5' read
  BG167325 cDNA clone IMAGE:4452656 hypernephroma, cell line 5' read
  BG501977 cDNA clone IMAGE:4654489 embryonal carcinoma 5' read
  BE744732 cDNA clone IMAGE:3834175 adenocarcinoma cell line 5' read
  BG776680 cDNA clone IMAGE:4809106 mucoepidermoid carcinoma 5' read Protein similarity
  BF673234 cDNA clone IMAGE:4272616 prostate 5' read
  AI653515 cDNA clone IMAGE:2216558 ovary 3' read 1.0 kbPolyadenylation signal
  F25985 cDNA clone s4000046E03 muscle Polyadenylation signal
  R40357 cDNA clone IMAGE:28705 brain 3' read 1.0 kbPolyadenylation signal

SENSE ESTs

  AW084051 cDNA clone IMAGE:2585364 moderately differentiated adenocarcinoma 3' read Protein similarityPolyadenylation signal
  AI436446 cDNA clone IMAGE:2129954 pancreas 3' read 0.9 kbProtein similarity
  AL036241 cDNA clone DKFZp564K012 brain 5' read
  AI702363 cDNA clone IMAGE:2293547 pancreas 3' read 2.3 kbPolyadenylation signal
  AI685061 cDNA clone IMAGE:2323670 pancreas 3' read 2.2 kbProtein similarityPolyadenylation signal
  AW512702 cDNA clone IMAGE:2703147 uterus 3' read Protein similarityPolyadenylation signal
  AV740155 cDNA clone CBLAIF12 cord blood 5' read Polyadenylation signal
  AF052498 cDNA clone RA49 lung Protein similarityPolyadenylation signal
  AI913301 cDNA clone IMAGE:2294486 pancreas 3' read 0.9 kbProtein similarityPolyadenylation signal
  AI432492 cDNA clone IMAGE:2120312 pancreas 3' read 0.9 kbPolyadenylation signal
  AU144532 cDNA clone HEMBA1002196 whole embryo, mainly head 3' read Protein similarityPolyadenylation signal
  AW513348 cDNA clone IMAGE:2706783 uterus 3' read Protein similarityPolyadenylation signal
  AW151208 cDNA clone IMAGE:2629454 uterus 3' read Protein similarityPolyadenylation signal
  BG032581 cDNA clone IMAGE:4392553 mammary adenocarcinoma, cell line 5' read Protein similarity
  AA385310 cDNA clone ATCC:189478 thyroid 5' read Protein similarity
  AU117795 cDNA clone HEMBA1002196 whole embryo, mainly head 5' read Protein similarity
  BI222368 cDNA clone IMAGE:5103123 choriocarcinoma 5' read Protein similarity
  AU138280 cDNA clone PLACE1008247 placenta 5' read Protein similarity
  BG180035 cDNA clone IMAGE:4430974 adenocarcinoma, cell line 5' read Protein similarity
  BF242046 cDNA clone IMAGE:4108443 from acute myelogenous leukemia 5' read