Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.25063
Description:PRO0461 protein
Best-Of-UniGene (BOU) SequenceNM_031268
Genomic Coordiantes Displayed:Bases 843000 to 857000 of contig Hs16_15516_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.25063.jpeg
Best sense EST/protein match:NM_031268 matched ref|NP_112558.1| (NM_031268) PRO0461 protein [Homo sapiens] (E = 7e-31)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AL039760 cDNA clone DKFZp434P1111 testis 5' read
  D58985 cDNA clone GEN518A04 placenta 5' read
  BG387508 cDNA clone IMAGE:4520813 embryonal carcinoma, cell line 5' read
  AA917385 cDNA clone IMAGE:1535898 kidney 3' read 0.7 kbPolyadenylation signal
  Z41180 cDNA clone c2qe05 brain 3' read Polyadenylation signal
  BG386334 cDNA clone IMAGE:4583793 adenocarcinoma cell line 5' read
  AL040505 cDNA clone DKFZp434G1114 testis 3' read
  AW275171 cDNA clone IMAGE:2819109 two pooled squamous cell carcinomas 3' read
  AA346395 cDNA clone ATCC:147860 heart 5' read
  AA341226 cDNA clone ATCC:143118 kidney 5' read
  BM129077 cDNA clone (no-name) islets of langerhans 5' read
  R36010 cDNA clone IMAGE:38478 brain 5' read 2.0 kb
  BG480872 cDNA clone IMAGE:4653266 choriocarcinoma 5' read