Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.263395
Description:sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Best-Of-UniGene (BOU) SequenceAK027867
Genomic Coordiantes Displayed:Bases 291933 to 475160 of contig Hs5_6630_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.263395.jpeg
Best sense EST/protein match:AK027867 matched ref|NP_065847.1| (NM_020796) semaphorin 6A1; sema domain, transmembrane domain (TM), (E = 0.0)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AI247563 cDNA clone IMAGE:1849084 pool 3' read 1.1 kbProtein similarity
  AI240716 cDNA clone IMAGE:1848074 pool 3' read 1.1 kb
  BG681304 cDNA clone IMAGE:4752085 squamous cell carcinoma 5' read
  AA682475 cDNA clone IMAGE:450634 pool 3' read Protein similarity
  AA700443 cDNA clone IMAGE:460610 pool 3' read Protein similarity