Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.26434
Description:hypothetical protein FLJ20360
Best-Of-UniGene (BOU) SequenceNM_017782
Genomic Coordiantes Displayed:Bases 6266000 to 6364000 of contig Hs10_8862_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.26434.jpeg
Best sense EST/protein match:AU123812 matched dbj|BAB14336.1| (AK022966) unnamed protein product [Homo sapiens] (E = e-153)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BF698482 cDNA clone IMAGE:4287497 primitive neuroectoderm 5' read
  BE866017 cDNA clone IMAGE:3960973 carcinoma, cell line 5' read
  AW070934 cDNA clone IMAGE:2568430 four pooled high-grade tumors, including two primary tumors 3' read 1.3 kbPolyadenylation signal
  AI079445 cDNA clone IMAGE:1680493 3' read 0.5 kbPolyadenylation signal
  AI335195 cDNA clone IMAGE:1915347 kidney 3' read 1.4 kbPolyadenylation signal
  AI923841 cDNA clone IMAGE:2451362 uterus 3' read Polyadenylation signal
  BG896983 cDNA clone (no-name) cartilage Polyadenylation signal
  AU153249 cDNA clone NT2RP3002726 3' read Polyadenylation signal
  AI978861 cDNA clone IMAGE:2492107 uterus 3' read 2.0 kbPolyadenylation signal
  AI358787 cDNA clone IMAGE:2005827 pancreas 3' read 2.9 kbPolyadenylation signal
  BG110214 cDNA clone IMAGE:4367486 osteosarcoma, cell line 5' read


  AL043619 cDNA clone DKFZp434H2127 testis 3' read Polyadenylation signal
  AI569072 cDNA clone IMAGE:2223200 pancreas 3' read 1.5 kbPolyadenylation signal
  AI701314 cDNA clone IMAGE:2323061 pancreas 3' read 4.3 kb
  AI640690 cDNA clone IMAGE:2294112 pancreas 3' read 1.7 kbPolyadenylation signal
  D62242 cDNA clone GEN249G09 aorta 5' read
  N67945 cDNA clone IMAGE:286761 ear 3' read
  D63021 cDNA clone GEN352G06 aorta 5' read
  AA720670 cDNA clone IMAGE:1241383 tonsil 3' read
  AW338816 cDNA clone IMAGE:2877899 pancreas 3' read Polyadenylation signal
  AW440981 cDNA clone IMAGE:2918270 myeloid cells, 18 pooled cml cases, bcr/abl rearrangement 3' read Polyadenylation signal
  AW337551 cDNA clone IMAGE:2847954 lymph 3' read
  AW080693 cDNA clone IMAGE:2585478 moderately differentiated adenocarcinoma 3' read
  AI888798 cDNA clone IMAGE:2447563 stomach 3' read 1.0 kb
  AI581196 cDNA clone IMAGE:2154779 colon 3' read 1.8 kbPolyadenylation signal
  D62442 cDNA clone GEN285F11 aorta 5' read
  D61857 cDNA clone GEN216G07 aorta 5' read
  BG287650 cDNA clone IMAGE:4513530 transitional cell papilloma, cell line 5' read
  AU151084 cDNA clone NT2RP2004365 3' read
  AI925713 cDNA clone IMAGE:2457144 stomach 3' read 1.4 kbPolyadenylation signal
  BE620478 cDNA clone IMAGE:3886276 large cell carcinoma, undifferentiated 5' read
  AL041883 cDNA clone DKFZp434H0919 testis 5' read
  AU126591 cDNA clone NT2RP1001235 5' read
  BG678091 cDNA clone IMAGE:4750926 squamous cell carcinoma 5' read
  BE748580 cDNA clone IMAGE:3838643 from acute myelogenous leukemia 3' read
  BI222382 cDNA clone IMAGE:5102889 choriocarcinoma 5' read
  BF977365 cDNA clone IMAGE:4305540 melanotic melanoma, high mdr 5' read
  BF342557 cDNA clone IMAGE:4149763 glioblastoma with egfr amplification 5' read
  BE889849 cDNA clone IMAGE:3913248 leiomyosarcoma 5' read
  BE748165 cDNA clone IMAGE:3838643 from acute myelogenous leukemia 5' read
  AW958742 cDNA clone (no-name) Protein similarity
  AV726380 cDNA clone HTCBGA08 hypothalamus 5' read
  AU139357 cDNA clone PLACE1010434 placenta 5' read
  AA383177 cDNA clone ATCC:187434 testis 5' read Protein similarity
  AU123812 cDNA clone NT2RM2001092 5' read
  BM048818 cDNA clone IMAGE:5456561 carcinoma, cell line 5' read
  AA331971 cDNA clone ATCC:133599 whole embryo 5' read Protein similarity
  BG030192 cDNA clone IMAGE:4391708 mammary adenocarcinoma, cell line 5' read