Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.268024
Description:Homo sapiens, clone IMAGE:3873720, mRNA
Best-Of-UniGene (BOU) SequenceAL110282
Genomic Coordiantes Displayed:Bases 340286 to 399413 of contig Hs1_4695_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.268024.jpeg
Best sense EST/protein match:AL110282 matched ref|XP_033704.1| (XM_033704) hypothetical protein XP_033704 [Homo sapiens] (E = e-104)
Best antisense EST/protein match:BE869699 matched ref|XP_072098.1| (XM_072098) hypothetical protein XP_072098 [Homo sapiens] (E = 7e-44)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI434194 cDNA clone IMAGE:2132089 stomach 3' read 1.4 kb
  AI568308 cDNA clone IMAGE:2174716 lymph 3' read 3.2 kb
  R44736 cDNA clone IMAGE:34137 brain 3' read 1.4 kbPolyadenylation signal
  AA342636 cDNA clone ATCC:144366 spleen 3' read Polyadenylation signal
  AI241891 cDNA clone IMAGE:1977459 brain 3' read 1.8 kbPolyadenylation signal
  W87475 cDNA clone IMAGE:417133 pool 3' read 2.4 kb
  AI076831 cDNA clone IMAGE:1674060 pool 3' read 1.1 kbPolyadenylation signal
  AA678959 cDNA clone IMAGE:1156187 kidney 3' read Polyadenylation signal
  AI049656 cDNA clone IMAGE:1700315 kidney 3' read Polyadenylation signal
  AA600308 cDNA clone IMAGE:1069323 kidney 3' read 0.8 kbPolyadenylation signal
  AA599024 cDNA clone IMAGE:898422 kidney 3' read 3.1 kbPolyadenylation signal
  AW512793 cDNA clone IMAGE:2792499 uterus 3' read Polyadenylation signal
  BI963734 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  BI964000 cDNA clone (no-name) islets of langerhans 5' read Polyadenylation signal
  AI476005 cDNA clone IMAGE:2154952 colon 3' read 1.5 kbPolyadenylation signal
  AA873695 cDNA clone IMAGE:1325824 kidney 3' read Polyadenylation signal
  AA678680 cDNA clone IMAGE:1155410 kidney 3' read Polyadenylation signal
  AW513839 cDNA clone IMAGE:2707362 uterus 3' read Polyadenylation signal
  AI446012 cDNA clone IMAGE:2143244 pancreas 3' read 2.8 kbPolyadenylation signal
  BE043311 cDNA clone IMAGE:3000093 lymph 3' read Polyadenylation signal
  AA682598 cDNA clone IMAGE:450838 pool 3' read Polyadenylation signal
  AW591851 cDNA clone IMAGE:2850570 lymph 3' read
  AW305023 cDNA clone IMAGE:2826555 three pooled meningiomas 3' read
  AA653361 cDNA clone IMAGE:1127848 kidney 3' read
  BG230609 cDNA clone IMAGE:4143323 lymphocyte 3' read
  BF727423 cDNA clone by21c04 lens 5' read Polyadenylation signal
  BG756055 cDNA clone IMAGE:4856602 primary b-cells from tonsils (cell line) 5' read
  BI856427 cDNA clone IMAGE:5396567 mammary adenocarcinoma, cell line 5' read
  BF796049 cDNA clone IMAGE:4342265 lymphoma, cell line 5' read
  R29655 cDNA clone F11177D liver 5' read
  BG403656 cDNA clone IMAGE:4526370 transitional cell papilloma, cell line 5' read
  BI115825 cDNA clone IMAGE:5014580 small cell carcinoma 5' read
  AV703226 cDNA clone ADBAFD07 adrenal gland 5' read
  BE535300 cDNA clone IMAGE:3445162 placenta 5' read
  BI117997 cDNA clone IMAGE:5015893 small cell carcinoma 5' read
  BI913372 cDNA clone IMAGE:5243126 brain 5' read
  BE869699 cDNA clone IMAGE:3849507 adenocarcinoma 5' read
  BG825083 cDNA clone IMAGE:4900188 rhabdomyosarcoma 5' read
  BE390360 cDNA clone IMAGE:3613416 endometrium, adenocarcinoma cell line 5' read
  W87570 cDNA clone IMAGE:417133 pool 5' read 2.4 kb
  BE267183 cDNA clone IMAGE:3534490 small cell carcinoma 5' read
  BG740327 cDNA clone IMAGE:4780314 skin 5' read
  BG249428 cDNA clone IMAGE:4470009 hypernephroma, cell line 5' read
  BE257308 cDNA clone IMAGE:3344883 retinoblastoma 5' read
  BE783035 cDNA clone IMAGE:3873720 retinoblastoma 5' read
  BI906346 cDNA clone IMAGE:5212325 leukocyte 5' read Protein similarity
  BE548020 cDNA clone IMAGE:3458170 choriocarcinoma 5' read
  BE904397 cDNA clone IMAGE:3898443 epithelioid carcinoma 5' read Protein similarity
  BG758454 cDNA clone IMAGE:4853076 primary b-cells from tonsils (cell line) 5' read
  BF664472 cDNA clone IMAGE:4309876 primary b-cells from tonsils (cell line) 5' read Protein similarity
  BE539363 cDNA clone IMAGE:3451552 placenta 5' read
  AW247089 cDNA clone IMAGE:2822458 small cell carcinoma 5' read

SENSE ESTs

  BG163417 cDNA clone IMAGE:4446335 hypernephroma, cell line 5' read
  BG253524 cDNA clone IMAGE:4472605 adenocarcinoma, cell line 5' read
  BF029884 cDNA clone IMAGE:3827120 hypernephroma 5' read