Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.278439
Description:nucleolar protein 3 (apoptosis repressor with CARD domain)
Best-Of-UniGene (BOU) SequenceBC007594
Genomic Coordiantes Displayed:Bases 1572005 to 1586912 of contig Hs16_10635_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.278439.jpeg
Best sense EST/protein match:BC007594 matched gb|AAH07594.1|AAH07594 (BC007594) Similar to RIKEN cDNA 4931428F04 gene [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:BM010663 matched ref|NP_003937.1| (NM_003946) nucleolar protein 3 (apoptosis repressor with CARD (E = 5e-83)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI684179 cDNA clone IMAGE:2267538 uterus 3' read 1.0 kbProtein similarityPolyadenylation signal
  BI597992 cDNA clone IMAGE:5300137 hypothalamus, cell line 5' read
  BG722063 cDNA clone IMAGE:4830662 testis, cell line 5' read
  AW241280 cDNA clone IMAGE:2570606 five pooled sarcomas, including myxoid liposarcoma, solitary 3' read
  AW517761 cDNA clone IMAGE:2898558 stomach 3' read
  AI912351 cDNA clone IMAGE:2289507 uterus 3' read 1.1 kb
  AI950550 cDNA clone IMAGE:2547260 two pooled squamous cell carcinomas 3' read
  AA086393 cDNA clone IMAGE:562693 muscle 3' read
  AA769691 cDNA clone IMAGE:1324151 kidney 3' read 0.6 kb
  AW103579 cDNA clone IMAGE:2614043 brain 3' read
  AA526859 cDNA clone IMAGE:984319 prostate 3' read 0.4 kb
  AA962119 cDNA clone IMAGE:1600281 germ cell 3' read 0.5 kb
  AW512158 cDNA clone IMAGE:2805568 uterus 3' read
  F29805 cDNA clone s4000083H04 muscle
  BF569082 cDNA clone IMAGE:4300597 epithelioid carcinoma cell line 3' read Protein similarity
  BG929256 cDNA clone (no-name) cartilage
  BG819372 cDNA clone IMAGE:4932380 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  AA336765 cDNA clone ATCC:138534 uterus 5' read
  AA102249 cDNA clone IMAGE:562693 muscle 5' read Protein similarity
  F34459 cDNA clone s5000004H01 muscle Protein similarity
  F17545 cDNA clone NOTAVAIL02094
  BF568452 cDNA clone IMAGE:4300597 epithelioid carcinoma cell line 5' read Protein similarity
  BE735394 cDNA clone IMAGE:3639056 adenocarcinoma 5' read Protein similarity
  BM010101 cDNA clone IMAGE:5444860 amelanotic melanoma, cell line 5' read Protein similarity
  BG742915 cDNA clone IMAGE:4777272 skin 5' read Protein similarity
  BG323679 cDNA clone IMAGE:4559945 renal cell adenocarcinoma 5' read Protein similarity
  BF683766 cDNA clone IMAGE:4301411 leiomyosarcoma cell line 5' read Protein similarity
  BG423856 cDNA clone IMAGE:4589423 renal cell adenocarcinoma 5' read Protein similarity
  F23321 cDNA clone s4000063B01 muscle
  BM010663 cDNA clone IMAGE:5448387 amelanotic melanoma, cell line 5' read Protein similarity
  AA149753 cDNA clone IMAGE:588443 5' read 1.5 kbProtein similarity
  BE273147 cDNA clone IMAGE:3506011 renal cell adenocarcinoma 5' read Protein similarity
  BI753792 cDNA clone IMAGE:5198337 brain 5' read Protein similarity
  BI825794 cDNA clone IMAGE:5168210 medulla 5' read Protein similarity
  BG910173 cDNA clone IMAGE:4937804 anaplastic oligodendroglioma with 1p/19q loss 5' read

SENSE ESTs

  BI490627 cDNA clone IMAGE:5173374 pooled brain, lung, testis 3' read
  AA699299 cDNA clone IMAGE:432548 pool 3' read Polyadenylation signal
  BG425052 cDNA clone IMAGE:4591086 renal cell adenocarcinoma 5' read
  BG701053 cDNA clone IMAGE:4814753 hippocampus, cell line 5' read
  BG700126 cDNA clone IMAGE:4812417 hippocampus, cell line 5' read