Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.279841
Description:hypothetical protein FLJ10335
Best-Of-UniGene (BOU) SequenceNM_018062
Genomic Coordiantes Displayed:Bases 326000 to 354000 of contig Hs2_5546_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.279841.jpeg
Best sense EST/protein match:AU123628 matched ref|NP_060532.1| (NM_018062) hypothetical protein FLJ10335 [Homo sapiens] (E = e-133)
Best antisense EST/protein match:AL353985 matched ref|NP_006287.1| (NM_006296) vaccinia related kinase 2; Vaccinia-related kinase-2 (E = 2e-48)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AI249475 cDNA clone IMAGE:2000731 lymph 3' read 0.9 kbPolyadenylation signal
  BE964642 cDNA clone IMAGE:3885808 large cell carcinoma, undifferentiated 3' read
  BE965853 cDNA clone IMAGE:3895806 epithelioid carcinoma 3' read
  AI888383 cDNA clone IMAGE:2446987 stomach 3' read 1.2 kbPolyadenylation signal
  AL036075 cDNA clone DKFZp564F0423 brain 3' read Polyadenylation signal