Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.28077
Description:GDP-mannose pyrophosphorylase B
Best-Of-UniGene (BOU) SequenceAB058754
Genomic Coordiantes Displayed:Bases 116407 to 126396 of contig Hs3_22595_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.28077.jpeg
Best sense EST/protein match:BE889800 matched ref|NP_037466.2| (NM_013334) GDP-mannose pyrophosphorylase B, isoform 1; (E = e-126)
Best antisense EST/protein match:AU148747 matched dbj|BAB14139.1| (AK022627) unnamed protein product [Homo sapiens] (E = 3e-29)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AA352885 cDNA clone ATCC:153162 5' read
  F18610 cDNA clone NOTAVAIL03199
  AU148747 cDNA clone NT2RM4000848 3' read Polyadenylation signal
  AW168523 cDNA clone IMAGE:2652807 skin 3' read Polyadenylation signal
  AW072995 cDNA clone IMAGE:2569600 five pooled sarcomas, including myxoid liposarcoma, solitary 3' read Polyadenylation signal
  AW571979 cDNA clone IMAGE:2840197 uterus 3' read Polyadenylation signal
  F36958 cDNA clone sH5000023-0/A02 muscle
  AI623715 cDNA clone IMAGE:2230849 uterus 3' read 3.7 kb
  AA369718 cDNA clone ATCC:174298 placenta 5' read


  BG709349 cDNA clone IMAGE:4796032 hypothalamus, cell line 5' read
  BG752492 cDNA clone IMAGE:4874685 normal pigmented retinal epithelium 5' read Protein similarity
  AU134806 cDNA clone PLACE1000547 placenta 5' read Protein similarity
  AU142348 cDNA clone Y79AA1000186 5' read Protein similarity
  AU099321 cDNA clone HRC09113
  BI819160 cDNA clone IMAGE:5177919 pooled brain, lung, testis 5' read Protein similarity
  BM047922 cDNA clone IMAGE:5432588 carcinoma, cell line 5' read