Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.281043
Description:Human clone 23909 mRNA, partial cds
Best-Of-UniGene (BOU) SequenceU79304
Genomic Coordiantes Displayed:Bases 2203024 to 2356247 of contig Hs10_8999_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.281043.jpeg
Best sense EST/protein match:BI463348 matched ref|XP_031373.2| (XM_031373) hypothetical protein XP_031373 [Homo sapiens] (E = e-109)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  T66181 cDNA clone IMAGE:22076 brain 3' read 1.6 kb
  F09848 cDNA clone c37b11 brain 3' read
  R45412 cDNA clone IMAGE:35418 brain 3' read 3.1 kb
  BF941084 cDNA clone IMAGE:3280584 dorsal root ganglia 3' read
  R59079 cDNA clone IMAGE:42144 brain 3' read 2.1 kb
  H18436 cDNA clone IMAGE:50990 brain 3' read 1.6 kb


  AL046116 cDNA clone DKFZp434M1772 testis 5' read Protein similarity
  BF448024 cDNA clone IMAGE:4090399 sympathetic trunk 3' read
  BI754502 cDNA clone IMAGE:5193866 brain 5' read
  BG720118 cDNA clone IMAGE:4823554 testis, cell line 5' read
  T77509 cDNA clone IMAGE:23909 brain 5' read 2.1 kbProtein similarity
  BI463348 cDNA clone IMAGE:5270033 testis, cell line 5' read
  BI822270 cDNA clone IMAGE:5177593 pooled brain, lung, testis 5' read
  AA331813 cDNA clone ATCC:133460 whole embryo 5' read Protein similarity