Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.282093
Description:hypothetical protein FLJ21918
Best-Of-UniGene (BOU) SequenceNM_024939
Genomic Coordiantes Displayed:Bases 461000 to 489000 of contig Hs16_10635_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.282093.jpeg
Best sense EST/protein match:NM_024939 matched ref|NP_079215.1| (NM_024939) hypothetical protein FLJ21918 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:AA247855 matched ref|NP_004546.1| (NM_004555) nuclear factor of activated T-cells, cytoplasmic, (E = 8e-14)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BG898969 cDNA clone (no-name) cartilage
  AW630512 cDNA clone IMAGE:2969421 2 pooled high-grade transitional cell tumors 5' read Protein similarity
  AW512094 cDNA clone IMAGE:2848994 lymph 3' read Polyadenylation signal
  AI281855 cDNA clone IMAGE:1962261 colon 3' read 1.0 kbPolyadenylation signal
  AW263398 cDNA clone IMAGE:2757729 three pooled meningiomas 3' read Polyadenylation signal
  T25876 cDNA clone CDDIR3 3' read Polyadenylation signal
  AW276203 cDNA clone IMAGE:2759481 two pooled squamous cell carcinomas 3' read Polyadenylation signal
  BG742309 cDNA clone IMAGE:4776781 skin 5' read
  BG281153 cDNA clone IMAGE:4543839 melanotic melanoma 5' read
  D81944 cDNA clone GEN425D02 brain 5' read
  BG283936 cDNA clone IMAGE:4520112 adenocarcinoma, cell line 5' read
  BG249392 cDNA clone IMAGE:4414874 hypernephroma, cell line 5' read
  BF242600 cDNA clone IMAGE:4104539 from acute myelogenous leukemia 5' read
  AI248247 cDNA clone IMAGE:1850532 pool 3' read 1.3 kbPolyadenylation signal
  AW674615 cDNA clone IMAGE:2985063 placenta 3' read
  AV703526 cDNA clone ADBDBF06 adrenal gland 5' read
  AA382843 cDNA clone ATCC:107552 testis 5' read
  AA247855 cDNA clone (no-name) heart 5' read Protein similarity
  AA345588 cDNA clone ATCC:147227 gall bladder 5' read
  BI091912 cDNA clone IMAGE:4999966 placenta 5' read
  BI259123 cDNA clone IMAGE:5112440 choriocarcinoma 5' read
  BI192532 cDNA clone IMAGE:5088939 epithelioid carcinoma cell line 5' read
  BG698444 cDNA clone IMAGE:4801557 skin 5' read
  N87955 cDNA clone K0875 heart 5' read
  BE544362 cDNA clone IMAGE:3462734 choriocarcinoma 5' read
  BE546250 cDNA clone IMAGE:3457222 choriocarcinoma 5' read
  BM020347 cDNA clone IMAGE:5430570 astrocytoma grade iv, cell line 5' read
  BE388195 cDNA clone IMAGE:3621490 endometrium, adenocarcinoma cell line 5' read
  AA361166 cDNA clone ATCC:165549 lymph 5' read
  N87595 cDNA clone LL2239 heart 5' read


  BG721782 cDNA clone IMAGE:4827975 testis, cell line 5' read
  BE563268 cDNA clone IMAGE:3689611 adenocarcinoma 5' read Protein similarity
  BM008672 cDNA clone IMAGE:5422523 adenocarcinoma 5' read
  BE019885 cDNA clone IMAGE:3029988 adenocarcinoma cell line 5' read Protein similarity
  BE872463 cDNA clone IMAGE:3851265 adenocarcinoma 5' read Protein similarity
  BI254464 cDNA clone IMAGE:5115965 choriocarcinoma 5' read Protein similarity
  BF033248 cDNA clone IMAGE:3858264 adenocarcinoma 5' read
  BG740274 cDNA clone IMAGE:4775927 skin 5' read
  BE264645 cDNA clone IMAGE:3536219 small cell carcinoma 5' read
  BG675841 cDNA clone IMAGE:4747242 squamous cell carcinoma 5' read
  AI910214 cDNA clone (no-name) breast
  BF035343 cDNA clone IMAGE:3861852 adenocarcinoma 5' read
  AW515254 cDNA clone IMAGE:2809301 uterus 3' read Polyadenylation signal
  AW438820 cDNA clone IMAGE:2778153 uterus 3' read Polyadenylation signal
  T72212 cDNA clone IMAGE:85804 liver 5' read 0.5 kb
  BG254345 cDNA clone IMAGE:4477083 adenocarcinoma, cell line 5' read
  T72068 cDNA clone IMAGE:85804 liver 3' read 0.5 kb
  BI964877 cDNA clone (no-name) islets of langerhans 5' read
  AW591552 cDNA clone IMAGE:2837472 uterus 3' read
  BI838404 cDNA clone IMAGE:5222369 pooled pancreas and spleen 5' read
  AW873122 cDNA clone IMAGE:3120179 neuroblastoma 3' read
  AW439187 cDNA clone IMAGE:2779298 uterus 3' read