Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.282804
Description:Hermansky-Pudlak syndrome 3
Best-Of-UniGene (BOU) SequenceAY033141
Genomic Coordiantes Displayed:Bases -3348 to 71955 of contig Hs3_5773_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.282804.jpeg
Best sense EST/protein match:AV709878 matched dbj|BAB71221.1| (AK056575) unnamed protein product [Homo sapiens] (E = e-108)
Best antisense EST/protein match:BE392045 matched gb|AAF34175.1|AF202115_1 (AF202115) GPI-anchored ceruloplasmin [Rattus norvegicus] (E = 2e-14)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI638138 cDNA clone IMAGE:2232070 uterus 3' read 0.7 kbPolyadenylation signal
  BE788857 cDNA clone IMAGE:3878374 large cell carcinoma 5' read
  AI565477 cDNA clone IMAGE:2214756 uterus 3' read
  AI648610 cDNA clone IMAGE:2268241 uterus 3' read 2.1 kbPolyadenylation signal
  AI811804 cDNA clone IMAGE:2261398 uterus 3' read 2.0 kbPolyadenylation signal
  AW051040 cDNA clone IMAGE:2557328 uterus 3' read Polyadenylation signal
  AA872345 cDNA clone IMAGE:1472653 kidney 3' read 1.4 kbPolyadenylation signal
  AW664198 cDNA clone IMAGE:2971470 2 pooled high-grade transitional cell tumors 3' read Polyadenylation signal
  AI934008 cDNA clone IMAGE:2453436 uterus 3' read Polyadenylation signal
  AI285677 cDNA clone IMAGE:1968209 lymph 3' read 1.2 kbPolyadenylation signal
  AI681978 cDNA clone IMAGE:2190723 uterus 3' read 1.4 kbPolyadenylation signal
  AI918520 cDNA clone IMAGE:2230296 uterus 3' read 2.5 kbPolyadenylation signal
  AI961576 cDNA clone IMAGE:2490890 uterus 3' read 1.0 kbPolyadenylation signal
  AW080243 cDNA clone IMAGE:2611288 uterus 3' read Polyadenylation signal
  AA872843 cDNA clone IMAGE:1471678 kidney 3' read 1.6 kb
  AI720659 cDNA clone IMAGE:2335431 colon 3' read
  BE392045 cDNA clone IMAGE:3625599 endometrium, adenocarcinoma cell line 5' read Protein similarity
  BF574193 cDNA clone IMAGE:4270884 muscle (skeletal) 5' read
  AI857292 cDNA clone IMAGE:2428286 uterus 3' read Polyadenylation signal
  AA769673 cDNA clone IMAGE:1324132 kidney 3' read 1.5 kbPolyadenylation signal
  BG563275 cDNA clone IMAGE:4710360 liver 5' read
  AI302101 cDNA clone IMAGE:1902406 kidney 3' read 0.6 kbPolyadenylation signal
  AI343881 cDNA clone IMAGE:1917840 kidney 3' read 0.6 kbPolyadenylation signal
  AI309991 cDNA clone IMAGE:1914521 kidney 3' read 1.4 kbPolyadenylation signal
  T40914 cDNA clone IMAGE:61422 liver 3' read Polyadenylation signal
  AA918982 cDNA clone IMAGE:1536240 kidney 3' read 1.2 kbPolyadenylation signal
  AA946849 cDNA clone IMAGE:1589127 kidney 3' read 0.6 kbPolyadenylation signal
  AI933892 cDNA clone IMAGE:2453403 uterus 3' read Polyadenylation signal
  AI690616 cDNA clone IMAGE:2277633 uterus 3' read 2.1 kbPolyadenylation signal
  AI648426 cDNA clone IMAGE:2274112 uterus 3' read 1.2 kbPolyadenylation signal
  AI889538 cDNA clone IMAGE:2444593 uterus 3' read Polyadenylation signal
  AI923375 cDNA clone IMAGE:2452154 uterus 3' read Polyadenylation signal
  AW088433 cDNA clone IMAGE:2593972 ovary 3' read Polyadenylation signal
  AI924724 cDNA clone IMAGE:2451617 uterus 3' read 1.4 kbPolyadenylation signal
  AW050523 cDNA clone IMAGE:2557946 uterus 3' read 1.2 kbPolyadenylation signal
  AU155184 cDNA clone OVARC1001213 ovary, tumor tissue 3' read Polyadenylation signal
  AA344899 cDNA clone ATCC:146417 gall bladder 3' read Polyadenylation signal
  T68167 cDNA clone IMAGE:83176 liver 3' read Polyadenylation signal
  BG618879 cDNA clone IMAGE:4767953 liver 5' read
  T69598 cDNA clone IMAGE:83560 liver 3' read Polyadenylation signal
  T41019 cDNA clone IMAGE:61599 liver 3' read
  BG618816 cDNA clone IMAGE:4767743 liver 5' read
  T67818 cDNA clone IMAGE:83043 liver 3' read
  BG617262 cDNA clone IMAGE:4734522 liver 5' read
  AA344900 cDNA clone ATCC:146417 gall bladder 5' read
  BG566593 cDNA clone IMAGE:4714352 liver 5' read
  BG563925 cDNA clone IMAGE:4712435 liver 5' read
  BG563706 cDNA clone IMAGE:4712323 liver 5' read
  BG568008 cDNA clone IMAGE:4715633 liver 5' read
  BG618581 cDNA clone IMAGE:4767720 liver 5' read
  BG618964 cDNA clone IMAGE:4767714 liver 5' read
  AI538280 cDNA clone IMAGE:2203812 uterus 3' read 0.9 kb
  AW193233 cDNA clone IMAGE:2680221 pancreas 3' read
  AI922198 cDNA clone IMAGE:2453011 uterus 3' read
  BG568287 cDNA clone IMAGE:4715811 liver 5' read
  AW303836 cDNA clone IMAGE:2761012 uterus 3' read
  T51148 cDNA clone IMAGE:78827 liver 3' read 0.4 kb
  AI985661 cDNA clone IMAGE:2507907 uterus 3' read 1.3 kb
  T71679 cDNA clone IMAGE:85316 liver 3' read
  BG567543 cDNA clone IMAGE:4715276 liver 5' read
  BG565366 cDNA clone IMAGE:4711323 liver 5' read
  BG618578 cDNA clone IMAGE:4767047 liver 5' read
  BG563030 cDNA clone IMAGE:4709786 liver 5' read

SENSE ESTs

  BG709275 cDNA clone IMAGE:4797256 hypothalamus, cell line 5' read
  BG722573 cDNA clone IMAGE:4826650 testis, cell line 5' read
  BG107069 cDNA clone IMAGE:4385940 lymphoma, cell line 5' read
  BI463041 cDNA clone IMAGE:5270392 testis, cell line 5' read
  BI906869 cDNA clone IMAGE:5213534 leukocyte 5' read
  BE884584 cDNA clone IMAGE:3907964 leiomyosarcoma 5' read
  BI770141 cDNA clone IMAGE:5202823 pooled lung and spleen 5' read
  BE778468 cDNA clone IMAGE:3867437 retinoblastoma 5' read
  BI090287 cDNA clone IMAGE:4996554 placenta 5' read
  BG261219 cDNA clone IMAGE:4484485 transitional cell papilloma, cell line 5' read
  BI760253 cDNA clone IMAGE:5185630 pooled colon, kidney, stomach 5' read
  BE267610 cDNA clone IMAGE:2989325 burkitt lymphoma 5' read
  BG163294 cDNA clone IMAGE:4446888 hypernephroma, cell line 5' read
  BI091876 cDNA clone IMAGE:5000052 placenta 5' read
  AA091140 cDNA clone (no-name) heart 5' read
  AI800359 cDNA clone IMAGE:2264453 uterus 3' read 1.4 kbPolyadenylation signal
  AW151423 cDNA clone IMAGE:2613649 uterus 3' read Polyadenylation signal
  BI223829 cDNA clone IMAGE:5105721 choriocarcinoma 5' read
  AA374183 cDNA clone ATCC:178565 5' read
  BG249519 cDNA clone IMAGE:4415076 hypernephroma, cell line 5' read
  BI224589 cDNA clone IMAGE:5104401 choriocarcinoma 5' read
  AI498559 cDNA clone IMAGE:2133987 lymph 3' read 0.5 kbPolyadenylation signal
  R21301 cDNA clone IMAGE:35798 brain 5' read 1.6 kb
  BE881946 cDNA clone IMAGE:3906419 leiomyosarcoma 5' read
  N87623 cDNA clone LL2314 heart 5' read
  BI860083 cDNA clone IMAGE:5396151 mammary adenocarcinoma, cell line 5' read
  AV709878 cDNA clone ADCALD03 adrenal gland 5' read
  BF571293 cDNA clone IMAGE:4251964 melanotic melanoma, high mdr 5' read
  BE513666 cDNA clone IMAGE:3634631 burkitt lymphoma 5' read
  BE567199 cDNA clone IMAGE:3683302 carcinoma, cell line 5' read
  BG716431 cDNA clone IMAGE:4798881 hypothalamus, cell line 5' read
  AV734860 cDNA clone cdAAPC06 pheochromocytoma 5' read
  BG179663 cDNA clone IMAGE:4430186 adenocarcinoma, cell line 5' read
  AA219354 cDNA clone IMAGE:650456 3' read Polyadenylation signal
  AW089848 cDNA clone IMAGE:2594489 ovary 3' read Polyadenylation signal
  AW474149 cDNA clone IMAGE:2852855 lymph 3' read Polyadenylation signal
  AI886539 cDNA clone IMAGE:2440363 uterus 3' read Polyadenylation signal
  R46148 cDNA clone IMAGE:35798 brain 3' read 1.6 kbPolyadenylation signal
  AI926046 cDNA clone IMAGE:2457895 stomach 3' read 0.3 kbPolyadenylation signal