Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.283652
Description:uncharacterized hypothalamus protein HT009
Best-Of-UniGene (BOU) SequenceNM_018470
Genomic Coordiantes Displayed:Bases 573482 to 609359 of contig Hs10_24233_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.283652.jpeg
Best sense EST/protein match:NM_018470 matched ref|NP_060940.1| (NM_018470) uncharacterized hypothalamus protein HT009 [Homo (E = e-105)
Best antisense EST/protein match:BF797297 matched ref|NP_004499.1| (NM_004508) isopentenyl-diphosphate delta isomerase; IPP isomerase (E = 3e-68)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF571443 cDNA clone IMAGE:4243948 melanotic melanoma, high mdr 5' read Protein similarity
  BF692855 cDNA clone IMAGE:4244441 muscle (skeletal) 5' read Protein similarity
  BF699009 cDNA clone IMAGE:4283403 primitive neuroectoderm 5' read Protein similarity
  BF695615 cDNA clone IMAGE:4076312 primitive neuroectoderm 5' read Protein similarity
  BF797297 cDNA clone IMAGE:4340570 lymphoma, cell line 5' read Protein similarity
  BE567060 cDNA clone IMAGE:3683381 carcinoma, cell line 5' read Protein similarity
  BG687159 cDNA clone IMAGE:4762701 mucoepidermoid carcinoma 5' read Protein similarity
  BF681067 cDNA clone IMAGE:4296450 prostate 5' read Protein similarity
  BF130676 cDNA clone IMAGE:4051386 hypernephroma 5' read Protein similarity
  BF243461 cDNA clone IMAGE:4106491 from acute myelogenous leukemia 5' read Protein similarity
  AV695563 cDNA clone GKCALB02 hepatocellular carcinoma 5' read Protein similarity
  AV698842 cDNA clone GKCALE06 hepatocellular carcinoma 5' read Protein similarity