Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.288835
Description:cell death-inducing DFFA-like effector b
Best-Of-UniGene (BOU) SequenceNM_014430
Genomic Coordiantes Displayed:Bases 2108000 to 2122000 of contig Hs14_19739_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.288835.jpeg
Best sense EST/protein match:NM_014430 matched ref|NP_055245.1| (NM_014430) cell death-inducing DFFA-like effector b [Homo sapiens] (E = e-109)
Best antisense EST/protein match:AA224262 matched dbj|BAB29900.1| (AK015585) Pumilio-family RNA binding domains (aka PUM-HD, Pumilio (E = 1e-19)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA602779 cDNA clone IMAGE:1116600 breast 3' read 0.7 kb
  BG253729 cDNA clone IMAGE:4474657 adenocarcinoma, cell line 5' read
  R16261 cDNA clone IMAGE:53082 brain 3' read Polyadenylation signal
  BM042804 cDNA clone IMAGE:5556939 melanotic melanoma, cell line 3' read
  BI871132 cDNA clone IMAGE:5404708 adenocarcinoma, cell line 5' read Polyadenylation signal
  BF795939 cDNA clone IMAGE:4342487 lymphoma, cell line 5' read
  BM042394 cDNA clone IMAGE:5556939 melanotic melanoma, cell line 5' read
  BE545429 cDNA clone IMAGE:3458986 choriocarcinoma 5' read
  BE788206 cDNA clone IMAGE:3884657 large cell carcinoma 5' read
  AI983821 cDNA clone IMAGE:2510965 pancreas 3' read 1.1 kb
  AA776513 cDNA clone IMAGE:1156371 kidney 3' read
  AA074157 cDNA clone IMAGE:531529 3' read
  AA161218 cDNA clone IMAGE:591204 3' read 0.6 kb
  Z41293 cDNA clone czpf08 brain 3' read
  AI579939 cDNA clone IMAGE:2210797 uterus 3' read 1.1 kb
  AA009760 cDNA clone IMAGE:429832 pool 3' read 1.9 kb
  BG337608 cDNA clone IMAGE:4553317 leiomyosarcoma cell line 5' read Protein similarity
  BE272678 cDNA clone IMAGE:3347820 renal cell adenocarcinoma 5' read
  BE272784 cDNA clone IMAGE:3347829 renal cell adenocarcinoma 5' read
  BG536876 cDNA clone IMAGE:4689388 lung 5' read Protein similarity
  BG539792 cDNA clone IMAGE:4692064 lung 5' read Protein similarity
  BE268927 cDNA clone IMAGE:3143175 burkitt lymphoma 5' read Protein similarity
  BE268907 cDNA clone IMAGE:3143222 burkitt lymphoma 5' read Protein similarity
  BE873058 cDNA clone IMAGE:3854876 adenocarcinoma 5' read Protein similarity
  BF530679 cDNA clone IMAGE:4214685 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BG112300 cDNA clone IMAGE:4369909 osteosarcoma, cell line 5' read Protein similarity
  BG681933 cDNA clone IMAGE:4754634 squamous cell carcinoma 5' read Protein similarity
  BM013755 cDNA clone IMAGE:5415626 mammary adenocarcinoma, cell line 5' read
  BI259928 cDNA clone IMAGE:5108275 choriocarcinoma 5' read Protein similarity
  BG291846 cDNA clone IMAGE:4515258 transitional cell papilloma, cell line 5' read
  BF345354 cDNA clone IMAGE:4154982 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BE791513 cDNA clone IMAGE:3940383 small cell carcinoma 5' read
  Z45614 cDNA clone czpf08 brain
  BE795932 cDNA clone IMAGE:3945143 small cell carcinoma 5' read
  AA322416 cDNA clone ATCC:122925 brain 5' read
  AA312349 cDNA clone ATCC:159517 5' read
  AA074483 cDNA clone IMAGE:531529 5' read
  R15446 cDNA clone IMAGE:53082 brain 5' read
  BE787960 cDNA clone IMAGE:3885487 large cell carcinoma 5' read Protein similarity
  BE792777 cDNA clone IMAGE:3939200 small cell carcinoma 5' read Protein similarity
  AA009759 cDNA clone IMAGE:429832 pool 5' read 1.9 kbProtein similarity
  BG752524 cDNA clone IMAGE:4874503 normal pigmented retinal epithelium 5' read Protein similarity
  BG420177 cDNA clone IMAGE:4590858 renal cell adenocarcinoma 5' read Protein similarity
  BE541081 cDNA clone IMAGE:3449908 placenta 5' read Protein similarity
  BE384837 cDNA clone IMAGE:3617306 melanotic melanoma 5' read Protein similarity
  BG392924 cDNA clone IMAGE:4540209 embryonal carcinoma, cell line 5' read
  AA224262 cDNA clone IMAGE:663442 5' read

SENSE ESTs

  BI836956 cDNA clone IMAGE:5223330 pooled pancreas and spleen 5' read Protein similarity
  BG706442 cDNA clone IMAGE:4792388 hypothalamus, cell line 5' read Protein similarity
  BG568535 cDNA clone IMAGE:4716269 liver 5' read Protein similarity
  BG562967 cDNA clone IMAGE:4708881 liver 5' read Protein similarity
  T50842 cDNA clone IMAGE:72785 spleen 5' read 0.9 kbProtein similarity
  BG564311 cDNA clone IMAGE:4723927 liver 5' read Protein similarity
  BG566147 cDNA clone IMAGE:4710619 liver 5' read Protein similarity
  AI051238 cDNA clone IMAGE:1673110 pool 3' read 1.2 kbPolyadenylation signal
  AI859599 cDNA clone IMAGE:2435883 uterus 3' read Polyadenylation signal
  T50687 cDNA clone IMAGE:72785 spleen 3' read 0.9 kbPolyadenylation signal
  AI242048 cDNA clone IMAGE:1851086 pool 3' read 1.4 kbPolyadenylation signal
  AI311713 cDNA clone IMAGE:1916008 kidney 3' read 1.3 kbPolyadenylation signal
  BE301428 cDNA clone IMAGE:3047967 choriocarcinoma 3' read Polyadenylation signal
  AW571732 cDNA clone IMAGE:2839886 uterus 3' read Polyadenylation signal
  AW772665 cDNA clone IMAGE:2692520 liver 5' read Polyadenylation signal