Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.296178
Description:hypothetical protein FLJ22637
Best-Of-UniGene (BOU) SequenceNM_025165
Genomic Coordiantes Displayed:Bases 5563000 to 5577000 of contig Hs15_10351_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.296178.jpeg
Best sense EST/protein match:BE794384 matched ref|NP_079441.1| (NM_025165) hypothetical protein FLJ22637 [Homo sapiens] (E = e-105)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI983685 cDNA clone IMAGE:2510931 pancreas 3' read 0.9 kb
  R39308 cDNA clone IMAGE:24267 brain 3' read 1.6 kb
  F09608 cDNA clone c33h08 brain 3' read Protein similarity
  T65582 cDNA clone IMAGE:21727 brain 3' read 1.5 kb
  R58979 cDNA clone IMAGE:41240 brain 3' read 1.3 kb
  R51585 cDNA clone IMAGE:39230 brain 3' read 1.6 kbProtein similarity
  W46767 cDNA clone IMAGE:324375 3' read 0.6 kbProtein similarity
  BI225632 cDNA clone IMAGE:5093466 burkitt lymphoma 5' read
  BE514048 cDNA clone IMAGE:3634904 burkitt lymphoma 5' read
  BE269183 cDNA clone IMAGE:3542032 burkitt lymphoma 5' read

SENSE ESTs

  AW129443 cDNA clone IMAGE:2607173 uterus 3' read
  AA714743 cDNA clone IMAGE:1241956 tonsil 3' read 1.8 kb
  AI569936 cDNA clone IMAGE:2222469 pancreas 3' read 1.8 kbPolyadenylation signal
  AW512317 cDNA clone IMAGE:2849262 lymph 3' read
  AI955716 cDNA clone IMAGE:2509665 pancreas 3' read Polyadenylation signal
  AW129442 cDNA clone IMAGE:2607171 uterus 3' read Polyadenylation signal
  AW246718 cDNA clone IMAGE:2821972 small cell carcinoma 3' read
  BM128884 cDNA clone (no-name) islets of langerhans 3' read
  BG777675 cDNA clone IMAGE:4804919 adenocarcinoma 5' read Protein similarity
  BM129151 cDNA clone (no-name) islets of langerhans 5' read
  AW129444 cDNA clone IMAGE:2607175 uterus 3' read Protein similarity
  AI978616 cDNA clone IMAGE:2491790 uterus 3' read 2.2 kb
  BG758722 cDNA clone IMAGE:4852966 primary b-cells from tonsils (cell line) 5' read
  BI093724 cDNA clone IMAGE:5001727 placenta 5' read
  BI771179 cDNA clone IMAGE:5204349 pooled lung and spleen 5' read Protein similarity
  BE794384 cDNA clone IMAGE:3943721 small cell carcinoma 5' read Protein similarity
  BF797851 cDNA clone IMAGE:4340600 lymphoma, cell line 5' read Protein similarity
  BG755008 cDNA clone IMAGE:4851823 primary b-cells from tonsils (cell line) 5' read Protein similarity
  BG685511 cDNA clone IMAGE:4765147 primary b-cells from tonsils (cell line) 5' read Protein similarity
  BI834594 cDNA clone IMAGE:5228650 pooled pancreas and spleen 5' read Protein similarity
  BG821699 cDNA clone IMAGE:4867323 adenocarcinoma cell line 5' read Protein similarity
  BI914135 cDNA clone IMAGE:5246204 brain 5' read Protein similarity
  BG341983 cDNA clone IMAGE:4575894 primary b-cells from tonsils (cell line) 5' read
  BG341970 cDNA clone IMAGE:4575652 primary b-cells from tonsils (cell line) 5' read Protein similarity
  BE798523 cDNA clone IMAGE:3937593 small cell carcinoma 5' read Protein similarity
  BE268650 cDNA clone IMAGE:3345598 burkitt lymphoma 5' read
  BE265796 cDNA clone IMAGE:3538386 small cell carcinoma 5' read
  BI550998 cDNA clone IMAGE:5275518 hippocampus, cell line 5' read
  AW250601 cDNA clone IMAGE:2821972 small cell carcinoma 5' read Protein similarity
  BI258528 cDNA clone IMAGE:5111744 choriocarcinoma 5' read Protein similarity
  BI462910 cDNA clone IMAGE:5270232 testis, cell line 5' read Protein similarity