Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.300870
Description:hypothetical gene DKFZp547M072
Best-Of-UniGene (BOU) SequenceAL512725
Genomic Coordiantes Displayed:Bases 150377 to 162589 of contig Hs19_11426_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.300870.jpeg
Best sense EST/protein match:BE903520 matched gb|AAH15089.1|AAH15089 (BC015089) Similar to midnolin [Homo sapiens] (E = 1e-51)
Best antisense EST/protein match:BI761921 matched ref|XP_072013.1| (XM_072013) hypothetical protein XP_072013 [Homo sapiens] (E = 5e-51)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA523193 cDNA clone IMAGE:979710 lung 3' read 1.1 kbPolyadenylation signal
  BI758813 cDNA clone IMAGE:5183310 pooled colon, kidney, stomach 5' read Protein similarity
  AA746590 cDNA clone IMAGE:1256442 germ cell 3' read 0.9 kb
  AI859904 cDNA clone IMAGE:2436765 uterus 3' read 2.2 kb
  AW172724 cDNA clone IMAGE:2656057 uterus 3' read
  R40087 cDNA clone IMAGE:27671 brain 3' read 1.7 kb
  AW440758 cDNA clone IMAGE:2918130 myeloid cells, 18 pooled cml cases, bcr/abl rearrangement 3' read Protein similarity
  AW589243 cDNA clone IMAGE:2920369 myeloid cells, 18 pooled cml cases, bcr/abl rearrangement 3' read
  BI762453 cDNA clone IMAGE:5188885 pooled colon, kidney, stomach 5' read
  BI761921 cDNA clone IMAGE:5188884 pooled colon, kidney, stomach 5' read
  BG223343 cDNA clone IMAGE:4170411 glioblastoma with probably tp53 mutation and without egfr 3' read

SENSE ESTs

  N69770 cDNA clone IMAGE:287387 ear 3' read Polyadenylation signal
  BG901151 cDNA clone (no-name) cartilage
  AA090880 cDNA clone (no-name) heart 5' read
  AW023784 cDNA clone IMAGE:2488129 ear 5' read
  T54530 cDNA clone IMAGE:73667 spleen 3' read 1.0 kbPolyadenylation signal
  N94567 cDNA clone IMAGE:309731 3' read 0.8 kb
  BF675346 cDNA clone IMAGE:4274689 prostate 5' read
  AI750054 cDNA clone IMAGE:2373981 colon 3' read Polyadenylation signal
  AI539744 cDNA clone IMAGE:2204834 uterus 3' read 1.4 kb
  AI581091 cDNA clone IMAGE:2142724 stomach 3' read 0.8 kb
  BF981016 cDNA clone IMAGE:4401531 duodenal adenocarcinoma, cell line 5' read
  AA328840 cDNA clone ATCC:130673 whole embryo 5' read
  AI142933 cDNA clone IMAGE:1679514 3' read
  BG222392 cDNA clone IMAGE:4170081 glioblastoma with probably tp53 mutation and without egfr 3' read
  W30858 cDNA clone IMAGE:309731 5' read 0.8 kb
  AI560206 cDNA clone IMAGE:2212416 uterus 3' read 2.2 kb
  AI627206 cDNA clone IMAGE:2283487 uterus 3' read 2.0 kb
  AI081510 cDNA clone IMAGE:1679107 3' read 1.0 kb
  T54569 cDNA clone IMAGE:73667 spleen 5' read 1.0 kb
  AI923834 cDNA clone IMAGE:2451341 uterus 3' read 0.9 kb
  AI300085 cDNA clone IMAGE:1902557 kidney 3' read 1.9 kb
  BF724422 cDNA clone bx04g01 iris 5' read
  AI079230 cDNA clone IMAGE:1678931 3' read 1.2 kb
  AA354325 cDNA clone ATCC:154567 5' read
  BE903641 cDNA clone IMAGE:3957867 choriocarcinoma 5' read
  BF338832 cDNA clone IMAGE:4184129 glioblastoma with egfr amplification 5' read
  BE281539 cDNA clone IMAGE:3138488 choriocarcinoma 5' read
  BG479050 cDNA clone IMAGE:4649838 choriocarcinoma 5' read
  BG925382 cDNA clone (no-name) cartilage
  BG420265 cDNA clone IMAGE:4587018 renal cell adenocarcinoma 5' read Protein similarity
  BG422282 cDNA clone IMAGE:4587174 renal cell adenocarcinoma 5' read Protein similarity
  BM012212 cDNA clone IMAGE:5458895 neuroblastoma, cell line 5' read
  BM012280 cDNA clone IMAGE:5458871 neuroblastoma, cell line 5' read