Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.302435
Description:centaurin, gamma 1
Best-Of-UniGene (BOU) SequenceNM_014770
Genomic Coordiantes Displayed:Bases 134451 to 159151 of contig Hs12_9567_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.302435.jpeg
Best sense EST/protein match:BF529135 matched ref|NP_055585.1| (NM_014770) KIAA0167 gene product [Homo sapiens] (E = e-104)
Best antisense EST/protein match:AW245733 matched gb|AAH19026.1|AAH19026 (BC019026) Unknown (protein for IMAGE:4558254) [Homo sapiens] (E = 3e-38)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BM009331 cDNA clone IMAGE:5443484 amelanotic melanoma, cell line 5' read Protein similarity
  BE264977 cDNA clone IMAGE:3537861 small cell carcinoma 5' read Protein similarity
  BM009830 cDNA clone IMAGE:5443990 amelanotic melanoma, cell line 5' read Protein similarity
  BG327113 cDNA clone IMAGE:4564257 renal cell adenocarcinoma 5' read Protein similarity
  BG423979 cDNA clone IMAGE:4586192 renal cell adenocarcinoma 5' read Protein similarity
  BE296133 cDNA clone IMAGE:3532453 rhabdomyosarcoma 5' read Protein similarity
  BG469657 cDNA clone IMAGE:4661743 adenocarcinoma cell line 5' read Protein similarity
  BE299434 cDNA clone IMAGE:3028600 rhabdomyosarcoma 5' read Protein similarity
  BG470569 cDNA clone IMAGE:4634297 retinoblastoma 5' read Protein similarity
  BF309969 cDNA clone IMAGE:4138068 rhabdomyosarcoma 5' read Protein similarity
  BG491431 cDNA clone IMAGE:4684783 amelanotic melanoma, cell line 5' read Protein similarity
  BI114377 cDNA clone IMAGE:5021428 rhabdomyosarcoma 5' read Protein similarity
  BI117754 cDNA clone IMAGE:5015439 small cell carcinoma 5' read Protein similarity
  BI753962 cDNA clone IMAGE:5193836 brain 5' read Protein similarity
  BI755479 cDNA clone IMAGE:5197998 brain 5' read Protein similarity
  AW245733 cDNA clone IMAGE:2822817 small cell carcinoma 5' read Protein similarity
  BI756796 cDNA clone IMAGE:5194960 brain 5' read Protein similarity
  BE251649 cDNA clone IMAGE:3351380 retinoblastoma 5' read Protein similarity
  BI914975 cDNA clone IMAGE:5247320 brain 5' read Protein similarity
  BE252847 cDNA clone IMAGE:3350835 retinoblastoma 5' read Protein similarity
  BE267340 cDNA clone IMAGE:3533459 small cell carcinoma 5' read Protein similarity
  BG622824 cDNA clone IMAGE:4769452 placenta 5' read Protein similarity
  BG170007 cDNA clone IMAGE:4426527 hypernephroma, cell line 5' read
  BF969704 cDNA clone IMAGE:4360200 adrenal cortex carcinoma, cell line 5' read Protein similarity
  AA622918 cDNA clone IMAGE:1118105 lung 3' read 1.6 kb
  AA352392 cDNA clone ATCC:152812 5' read Protein similarity

SENSE ESTs

  BI755875 cDNA clone IMAGE:5194646 brain 5' read
  BE280072 cDNA clone IMAGE:3505568 choriocarcinoma 5' read
  AL134589 cDNA clone DKFZp547D186 brain 5' read
  AL134787 cDNA clone DKFZp547K2490 brain 5' read Protein similarity
  BG825364 cDNA clone IMAGE:4900569 rhabdomyosarcoma 5' read Protein similarity
  AL120358 cDNA clone DKFZp761B038 amygdala 5' read Protein similarity
  AW087290 cDNA clone IMAGE:2582877 moderately differentiated adenocarcinoma 3' read Protein similarity
  BF529135 cDNA clone IMAGE:4179960 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BI911618 cDNA clone IMAGE:5213388 leukocyte 5' read Protein similarity