Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.309952
Description:Homo sapiens mRNA; cDNA DKFZp434E0516 (from clone DKFZp434E0516)
Best-Of-UniGene (BOU) SequenceAL137656
Genomic Coordiantes Displayed:Bases 1226814 to 1249322 of contig Hs19_11287_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.309952.jpeg
Best sense EST/protein match:AL137656 matched ref|XP_071980.1| (XM_071980) hypothetical protein XP_071980 [Homo sapiens] (E = 5e-70)
Best antisense EST/protein match:BF309986 matched dbj|BAB23670.1| (AK004919) data source:SPTR, source key:Q9HCJ3, (E = e-109)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BE257555 cDNA clone IMAGE:3350335 retinoblastoma 5' read
  BG752149 cDNA clone IMAGE:4875132 normal pigmented retinal epithelium 5' read Protein similarity
  BG468971 cDNA clone IMAGE:4645371 adenocarcinoma cell line 5' read Protein similarity
  BF127585 cDNA clone IMAGE:4053129 leiomyosarcoma cell line 5' read
  BG476369 cDNA clone IMAGE:4640489 melanotic melanoma 5' read Protein similarity
  BE313942 cDNA clone IMAGE:3162565 neuroblastoma 5' read Protein similarity
  BG106652 cDNA clone IMAGE:4385181 lymphoma, cell line 5' read Protein similarity
  BI255037 cDNA clone IMAGE:5113697 choriocarcinoma 5' read Protein similarity
  BF970177 cDNA clone IMAGE:4361708 adrenal cortex carcinoma, cell line 5' read Protein similarity
  BE885049 cDNA clone IMAGE:3912286 leiomyosarcoma 5' read
  BG911480 cDNA clone IMAGE:4940998 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BG032369 cDNA clone IMAGE:4403028 mammary adenocarcinoma, cell line 5' read Protein similarity
  BE385357 cDNA clone IMAGE:3618235 melanotic melanoma 5' read
  BE895245 cDNA clone IMAGE:3918693 melanotic melanoma 5' read Protein similarity
  BI256263 cDNA clone IMAGE:5114276 choriocarcinoma 5' read Protein similarity
  BF309986 cDNA clone IMAGE:4138168 rhabdomyosarcoma 5' read Protein similarity
  BF305885 cDNA clone IMAGE:4139057 rhabdomyosarcoma 5' read
  BF346256 cDNA clone IMAGE:4154146 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BF310750 cDNA clone IMAGE:4124986 neuroblastoma 5' read
  BE885742 cDNA clone IMAGE:3910476 leiomyosarcoma 5' read
  BG032400 cDNA clone IMAGE:4403132 mammary adenocarcinoma, cell line 5' read Protein similarity
  BI561260 cDNA clone IMAGE:5298794 testis, cell line 5' read Protein similarity
  BG825735 cDNA clone IMAGE:4900045 rhabdomyosarcoma 5' read Protein similarity
  BI333296 cDNA clone IMAGE:5138606 choriocarcinoma 5' read Protein similarity
  BF984117 cDNA clone IMAGE:4398192 duodenal adenocarcinoma, cell line 5' read Protein similarity
  BG751625 cDNA clone IMAGE:4874337 normal pigmented retinal epithelium 5' read Protein similarity
  BG772457 cDNA clone IMAGE:4839338 testis, cell line 5' read Protein similarity
  BG257988 cDNA clone IMAGE:4509997 embryonal carcinoma, cell line 5' read Protein similarity
  BE299234 cDNA clone IMAGE:3028195 rhabdomyosarcoma 5' read Protein similarity
  BI335547 cDNA clone IMAGE:5139841 choriocarcinoma 5' read
  BE298434 cDNA clone IMAGE:3029119 rhabdomyosarcoma 5' read Protein similarity
  BF204126 cDNA clone IMAGE:4111553 rhabdomyosarcoma 5' read
  BI260283 cDNA clone IMAGE:5112139 choriocarcinoma 5' read Protein similarity
  BG324377 cDNA clone IMAGE:4561126 renal cell adenocarcinoma 5' read Protein similarity

SENSE ESTs

  BI828565 cDNA clone IMAGE:5170167 medulla 5' read
  BI828676 cDNA clone IMAGE:5170389 medulla 5' read Protein similarity
  BG333279 cDNA clone IMAGE:4549008 large cell carcinoma 5' read Protein similarity
  BG331450 cDNA clone IMAGE:4550745 large cell carcinoma 5' read Protein similarity
  BG028801 cDNA clone IMAGE:4388209 osteosarcoma, cell line 5' read Protein similarity
  BG110432 cDNA clone IMAGE:4366355 osteosarcoma, cell line 5' read