Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.315689
Description:Homo sapiens cDNA: FLJ22373 fis, clone HRC06741
Best-Of-UniGene (BOU) SequenceAK057062
Genomic Coordiantes Displayed:Bases 1363600 to 1373400 of contig Hs22_11676_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.315689.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BF028311 cDNA clone IMAGE:3997175 carcinoma, cell line 5' read
  AI074411 cDNA clone IMAGE:1679230 3' read 2.1 kbPolyadenylation signal
  AI684235 cDNA clone IMAGE:2275894 uterus 3' read 1.2 kbPolyadenylation signal
  AW438817 cDNA clone IMAGE:2778164 uterus 3' read Polyadenylation signal
  AI302873 cDNA clone IMAGE:1901512 kidney 3' read
  AW572010 cDNA clone IMAGE:2850692 lymph 3' read Polyadenylation signal
  AI061241 cDNA clone IMAGE:1700742 kidney 3' read Polyadenylation signal
  AI572067 cDNA clone IMAGE:2223936 pancreas 3' read 1.5 kbPolyadenylation signal
  AI282959 cDNA clone IMAGE:1961815 colon 3' read 2.0 kbPolyadenylation signal
  AW090430 cDNA clone IMAGE:2590799 brain 3' read
  BI918273 cDNA clone IMAGE:5247203 brain 5' read
  BG026025 cDNA clone IMAGE:4386960 osteosarcoma, cell line 5' read
  BI767608 cDNA clone IMAGE:5210049 pooled lung and spleen 5' read
  BG528843 cDNA clone IMAGE:4713608 adenocarcinoma 5' read


  BG177115 cDNA clone IMAGE:4419087 lymphoma, cell line 5' read
  AV725354 cDNA clone HTCAUH08 hypothalamus 5' read
  AA360903 cDNA clone ATCC:165046 lymph 5' read
  BG620740 cDNA clone IMAGE:4731490 placenta 5' read
  BF105282 cDNA clone IMAGE:4042577 kidney 5' read
  BG696745 cDNA clone IMAGE:4802850 skin 5' read
  BI090912 cDNA clone IMAGE:5000432 placenta 5' read
  BE737294 cDNA clone IMAGE:3641551 adenocarcinoma 5' read
  AI872099 cDNA clone IMAGE:2439839 uterus 3' read 2.6 kbPolyadenylation signal