Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.32450
Description:Homo sapiens cDNA FLJ30846 fis, clone FEBRA2002783
Best-Of-UniGene (BOU) SequenceAK055408
Genomic Coordiantes Displayed:Bases 607580 to 706448 of contig Hs13_10074_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.32450.jpeg
Best sense EST/protein match:AK055408 matched ref|XP_058595.1| (XM_058595) similar to putative (H. sapiens) [Homo sapiens] (E = e-100)
Best antisense EST/protein match:BI561289 matched ref|NP_037410.1| (NM_013278) interleukin 17C [Homo sapiens] (E = 6e-13)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI601695 cDNA clone IMAGE:5301608 hypothalamus, cell line 5' read
  BG772779 cDNA clone IMAGE:4837692 testis, cell line 5' read
  BI561289 cDNA clone IMAGE:5298706 testis, cell line 5' read Protein similarity
  BI828970 cDNA clone IMAGE:5166851 medulla 5' read Protein similarity
  AA385051 cDNA clone ATCC:189212 thyroid 5' read
  BG109297 cDNA clone IMAGE:4368800 osteosarcoma, cell line 5' read
  BM128162 cDNA clone (no-name) islets of langerhans 5' read
  BI827635 cDNA clone IMAGE:5166144 medulla 5' read
  F32964 cDNA clone s3000049H10 muscle
  AA303602 cDNA clone ATCC:115256 aorta 5' read
  F35345 cDNA clone sH3000006-3/C03 muscle
  BM127865 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  AA321825 cDNA clone ATCC:122361 brain 3' read Polyadenylation signal
  F17157 cDNA clone NOTAVAIL01678
  H29012 cDNA clone IMAGE:49936 brain 3' read 2.7 kbPolyadenylation signal
  F36829 cDNA clone sH5000020-0/B09 muscle

SENSE ESTs

  AI755175 cDNA clone HBMSC_cr36e07 bone 3' read Protein similarityPolyadenylation signal
  AA835551 cDNA clone IMAGE:1413751 spleen 3' read Polyadenylation signal
  AA693580 cDNA clone IMAGE:434333 pool 3' read Polyadenylation signal
  AI302819 cDNA clone IMAGE:1902487 kidney 3' read 2.7 kbProtein similarityPolyadenylation signal
  BG498110 cDNA clone IMAGE:4666059 adenocarcinoma 5' read Protein similarity
  AW189445 cDNA clone IMAGE:2675459 uterus 3' read Protein similarityPolyadenylation signal
  BI668445 cDNA clone IMAGE:5313151 hypothalamus, cell line 5' read Protein similarity
  AI302825 cDNA clone IMAGE:1902503 kidney 3' read 2.3 kbProtein similarityPolyadenylation signal
  BG283539 cDNA clone IMAGE:4520002 adenocarcinoma, cell line 5' read Protein similarity
  BG707022 cDNA clone IMAGE:4793121 hypothalamus, cell line 5' read Protein similarity
  BI600319 cDNA clone IMAGE:5302425 hypothalamus, cell line 5' read Protein similarity
  BE872173 cDNA clone IMAGE:3850364 adenocarcinoma 5' read Protein similarity
  BG403594 cDNA clone IMAGE:4526400 transitional cell papilloma, cell line 5' read Protein similarity
  AA705409 cDNA clone IMAGE:462159 pool 3' read Protein similarityPolyadenylation signal
  AA328362 cDNA clone ATCC:130019 whole embryo 5' read Protein similarity
  D79896 cDNA clone GEN352A04 aorta 5' read Protein similarity
  AW469466 cDNA clone IMAGE:2899450 stomach 3' read
  BE778999 cDNA clone IMAGE:3868232 retinoblastoma 5' read Protein similarity
  AA375974 cDNA clone ATCC:180418 5' read Protein similarity
  AL597222 cDNA clone DKFZp313O0511 5' read