Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.334479
Description:KIAA1306 protein
Best-Of-UniGene (BOU) SequenceAB037727
Genomic Coordiantes Displayed:Bases 176400 to 194600 of contig Hs16_10700_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.334479.jpeg
Best sense EST/protein match:AB037727 matched dbj|BAA92544.1| (AB037727) KIAA1306 protein [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:AI719689 matched ref|XP_058763.1| (XM_058763) hypothetical protein XP_058763 [Homo sapiens] (E = 3e-48)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AI587290 cDNA clone IMAGE:2207819 uterus 3' read 1.5 kbPolyadenylation signal
  AI955896 cDNA clone IMAGE:2509047 uterus 3' read 1.8 kb
  AI367407 cDNA clone IMAGE:1991457 uterus 3' read 0.9 kb
  AI521570 cDNA clone IMAGE:2183091 stomach 3' read 2.0 kbPolyadenylation signal
  BG033993 cDNA clone IMAGE:4402442 mammary adenocarcinoma, cell line 5' read
  AI953929 cDNA clone IMAGE:2549097 three pooled meningiomas 3' read
  BE618777 cDNA clone IMAGE:3866136 retinoblastoma 3' read
  AW243890 cDNA clone IMAGE:2708152 uterus 3' read
  AI367406 cDNA clone IMAGE:1991455 uterus 3' read 0.5 kb
  AW029015 cDNA clone IMAGE:2542212 stomach 3' read
  AW673873 cDNA clone IMAGE:2900765 placenta 3' read
  AA700002 cDNA clone IMAGE:436023 pool 3' read
  AA776309 cDNA clone IMAGE:453697 pool 3' read
  BI862451 cDNA clone IMAGE:5406257 mammary adenocarcinoma, cell line 5' read
  AI887590 cDNA clone IMAGE:2436031 uterus 3' read
  AA338685 cDNA clone ATCC:140500 brain 3' read
  AI709028 cDNA clone IMAGE:2332374 colon 3' read
  BM014797 cDNA clone IMAGE:5416697 mammary adenocarcinoma, cell line 5' read
  AI719689 cDNA clone IMAGE:2332886 colon 3' read
  AW084605 cDNA clone IMAGE:2569725 five pooled sarcomas, including myxoid liposarcoma, solitary 3' read
  AA070161 cDNA clone IMAGE:530913 3' read
  R43995 cDNA clone IMAGE:33432 brain 3' read 1.8 kb
  F37348 cDNA clone sH5000025-0/D12 muscle
  BE777833 cDNA clone IMAGE:3866684 retinoblastoma 5' read
  F27478 cDNA clone s4000071B08 muscle
  BE270970 cDNA clone IMAGE:2966610 burkitt lymphoma 3' read
  BE271146 cDNA clone IMAGE:2966484 burkitt lymphoma 3' read
  BG744794 cDNA clone IMAGE:4849396 natural killer cells, cell line 3' read
  BG683926 cDNA clone IMAGE:4761720 neuroblastoma, cell line 3' read