Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.334489
Description:Src-like-adaptor 2
Best-Of-UniGene (BOU) SequenceNM_032214
Genomic Coordiantes Displayed:Bases 279061 to 335541 of contig Hs20_11519_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.334489.jpeg
Best sense EST/protein match:BG284179 matched ref|NP_115590.1| (NM_032214) Src-like adapter protein-2; src-like-adaptor 2 [Homo (E = 4e-65)
Best antisense EST/protein match:BI962930 matched ref|NP_061328.1| (NM_018840) putative Rab5-interacting protein [Homo sapiens] (E = 2e-68)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BE543050 cDNA clone IMAGE:3455370 choriocarcinoma 5' read Protein similarity
  BG900162 cDNA clone (no-name) cartilage
  BE786629 cDNA clone IMAGE:3881535 large cell carcinoma 5' read
  BG281631 cDNA clone IMAGE:4544809 melanotic melanoma 5' read
  BE277112 cDNA clone IMAGE:3544012 melanotic melanoma 5' read Protein similarity
  BE277290 cDNA clone IMAGE:3546888 melanotic melanoma 5' read Protein similarity
  BG256022 cDNA clone IMAGE:4475499 adenocarcinoma, cell line 5' read
  BI962930 cDNA clone (no-name) islets of langerhans 5' read Protein similarity