Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.334772
Description:hypothetical protein FLJ13614
Best-Of-UniGene (BOU) SequenceNM_032182
Genomic Coordiantes Displayed:Bases 1040000 to 1110000 of contig Hs4_6265_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.334772.jpeg
Best sense EST/protein match:BG570593 matched dbj|BAB14189.1| (AK022704) unnamed protein product [Homo sapiens] (E = 3e-89)
Best antisense EST/protein match:BE874586 matched ref|NP_057151.1| (NM_016067) mitochondrial ribosomal protein S18C; CGI-134 protein; (E = 1e-60)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BI912725 cDNA clone IMAGE:5240564 brain 5' read
  AU144027 cDNA clone HEMBA1000684 whole embryo, mainly head 3' read Polyadenylation signal
  F08936 cDNA clone c2ug08 brain 3' read Polyadenylation signal
  F20292 cDNA clone NOTAVAIL04528 muscle Protein similarity
  AV757321 cDNA clone BMFARF02 bone marrow 5' read Protein similarityPolyadenylation signal
  BE874586 cDNA clone IMAGE:3888433 large cell carcinoma, undifferentiated 5' read
  F30306 cDNA clone s4000098F03 muscle Protein similarity


  AU125653 cDNA clone NT2RM4001965 5' read
  BI462610 cDNA clone IMAGE:5269087 testis, cell line 5' read
  AL599192 cDNA clone DKFZp313P0324 5' read Protein similarity
  AU139555 cDNA clone PLACE1010857 placenta 5' read
  AL602519 cDNA clone DKFZp686C1814 human skeletal muscle 5' read
  BI909258 cDNA clone IMAGE:5216239 leukocyte 5' read
  BI771321 cDNA clone IMAGE:5204182 pooled lung and spleen 5' read
  BE894160 cDNA clone IMAGE:3923326 melanotic melanoma 5' read
  BE888265 cDNA clone IMAGE:3913172 leiomyosarcoma 5' read
  BG570593 cDNA clone IMAGE:4717987 lung 5' read
  BI464110 cDNA clone IMAGE:5268502 testis, cell line 5' read
  AU117870 cDNA clone HEMBA1002381 whole embryo, mainly head 5' read Protein similarity
  BG531177 cDNA clone IMAGE:4699294 embryonal carcinoma 5' read
  AI830957 cDNA clone IMAGE:2409123 lymph 3' read 1.2 kb
  AU144581 cDNA clone HEMBA1002381 whole embryo, mainly head 3' read
  AW467496 cDNA clone IMAGE:2919477 myeloid cells, 18 pooled cml cases, bcr/abl rearrangement 3' read Polyadenylation signal
  BF240591 cDNA clone IMAGE:4099563 from acute myelogenous leukemia 5' read
  AI472709 cDNA clone IMAGE:2044067 lymph 3' read
  W51769 cDNA clone IMAGE:324469 3' read 0.8 kbPolyadenylation signal
  BF218805 cDNA clone IMAGE:4094834 glioblastoma 5' read
  BG494352 cDNA clone IMAGE:4660416 mucoepidermoid carcinoma 5' read
  BG776096 cDNA clone IMAGE:4808386 mucoepidermoid carcinoma 5' read