Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.38774
Description:Homo sapiens PBDX-like protein mRNA, complete cds
Best-Of-UniGene (BOU) SequenceAF380356
Genomic Coordiantes Displayed:Bases 183301 to 284073 of contig HsX_25446_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.38774.jpeg
Best sense EST/protein match:AF380356 matched sp|P55808|XG_HUMAN XG GLYCOPROTEIN PRECURSOR (PROTEIN PBDX) (E = 3e-59)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF248492 cDNA clone IMAGE:4046178 melanotic melanoma, high mdr 5' read
  BF979038 cDNA clone IMAGE:4306688 melanotic melanoma, high mdr 5' read
  BF978157 cDNA clone IMAGE:4307181 melanotic melanoma, high mdr 5' read
  BF977727 cDNA clone IMAGE:4307506 melanotic melanoma, high mdr 5' read
  BF572844 cDNA clone IMAGE:4253978 melanotic melanoma, high mdr 5' read

SENSE ESTs

  BF718591 cDNA clone S90328.NIH436-R epidermis 3' read Protein similarity
  AL599145 cDNA clone DKFZp313L1624 5' read Protein similarity
  AA337667 cDNA clone ATCC:139418 uterus 5' read Protein similarity
  AA336889 cDNA clone ATCC:138633 uterus 5' read Protein similarity