Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.4187
Description:hypothetical protein 24636
Best-Of-UniGene (BOU) SequenceAF070643
Genomic Coordiantes Displayed:Bases 1363156 to 1373658 of contig Hs17_10965_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.4187.jpeg
Best sense EST/protein match:BI713151 matched ref|XP_031534.2| (XM_031534) hypothetical protein XP_031534 [Homo sapiens] (E = 6e-55)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AL134957 cDNA clone DKFZp762D0711 melanoma (mewo cell line) 5' read
  R39259 cDNA clone IMAGE:23154 brain 3' read 1.8 kb
  AU150450 cDNA clone NT2RP2002813 3' read
  AW273409 cDNA clone IMAGE:2762473 uterus 3' read
  AU151597 cDNA clone NT2RP2005659 3' read
  AI248290 cDNA clone IMAGE:1849528 pool 3' read 1.1 kb
  T09998 cDNA clone b4HB3MACOT8-HAP-Ft5 brain 3' read
  BG329422 cDNA clone IMAGE:4558240 retinoblastoma 5' read
  AU150186 cDNA clone NT2RP2001860 3' read
  F10591 cDNA clone c3hc09 brain 3' read Polyadenylation signal

SENSE ESTs

  BG429132 cDNA clone IMAGE:4607995 kidney 5' read
  BG250010 cDNA clone IMAGE:4470762 adenocarcinoma, cell line 5' read
  BI548635 cDNA clone IMAGE:5261061 hippocampus, cell line 5' read
  BI869442 cDNA clone IMAGE:5403134 adenocarcinoma, cell line 5' read
  BI870777 cDNA clone IMAGE:5402975 adenocarcinoma, cell line 5' read
  BF205812 cDNA clone IMAGE:4110867 rhabdomyosarcoma 5' read
  BG034882 cDNA clone IMAGE:4413513 adenocarcinoma, cell line 5' read
  BI551117 cDNA clone IMAGE:5275702 hippocampus, cell line 5' read
  BE379074 cDNA clone IMAGE:3609552 endometrium, adenocarcinoma cell line 5' read Protein similarity
  BE305254 cDNA clone IMAGE:3528650 rhabdomyosarcoma 5' read
  AA159430 cDNA clone IMAGE:591419 5' read
  BG328223 cDNA clone IMAGE:4547069 adenocarcinoma cell line 5' read
  BI918835 cDNA clone IMAGE:5244663 brain 5' read
  BI550163 cDNA clone IMAGE:5264483 hippocampus, cell line 5' read
  BG386457 cDNA clone IMAGE:4584021 adenocarcinoma cell line 5' read
  BI713151 cDNA clone (no-name) purified pancreatic islet 5' read
  BI755072 cDNA clone IMAGE:5194019 brain 5' read
  BI755076 cDNA clone IMAGE:5193925 brain 5' read
  BE870133 cDNA clone IMAGE:3853954 adenocarcinoma 5' read
  BE619475 cDNA clone IMAGE:3876314 large cell carcinoma 5' read
  BG536336 cDNA clone IMAGE:4689537 lung 5' read
  BE884392 cDNA clone IMAGE:3907361 leiomyosarcoma 5' read
  W24129 cDNA clone IMAGE:310008 5' read 0.8 kb
  BE619798 cDNA clone IMAGE:3876314 large cell carcinoma 3' read