Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.44268
Description:myelin gene expression factor 2
Best-Of-UniGene (BOU) SequenceAB037762
Genomic Coordiantes Displayed:Bases 1970797 to 2026503 of contig Hs15_28523_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.44268.jpeg
Best sense EST/protein match:AU130981 matched dbj|BAB14421.1| (AK023133) unnamed protein product [Homo sapiens] (E = 3e-97)
Best antisense EST/protein match:BI858597 matched ref|NP_065740.1| (NM_020689) sodium calcium exchanger [Homo sapiens] (E = 2e-42)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI858597 cDNA clone IMAGE:5397950 mammary adenocarcinoma, cell line 5' read Protein similarity
  BF979157 cDNA clone IMAGE:4306725 melanotic melanoma, high mdr 5' read
  BF692141 cDNA clone IMAGE:4333929 melanotic melanoma, high mdr 5' read Protein similarity
  BF691934 cDNA clone IMAGE:4334028 melanotic melanoma, high mdr 5' read Protein similarity
  BE961022 cDNA clone IMAGE:3932324 melanotic melanoma, high mdr 5' read Protein similarity
  AW263476 cDNA clone IMAGE:2757900 three pooled meningiomas 3' read Polyadenylation signal
  AI969605 cDNA clone IMAGE:2563300 malignant melanoma, metastatic to lymph node 3' read 2.4 kbProtein similarity
  BE221473 cDNA clone IMAGE:3171114 malignant melanoma, metastatic to lymph node 3' read Protein similarityPolyadenylation signal
  BE327994 cDNA clone IMAGE:3171659 malignant melanoma, metastatic to lymph node 3' read Polyadenylation signal

SENSE ESTs

  AI445887 cDNA clone IMAGE:2140742 stomach 3' read 1.4 kb
  BE868308 cDNA clone IMAGE:3848242 adenocarcinoma 5' read Protein similarity
  BG818024 cDNA clone IMAGE:4915084 anaplastic oligodendroglioma with 1p/19q loss 5' read
  AI439426 cDNA clone IMAGE:2140513 stomach 3' read 1.3 kbPolyadenylation signal
  AW023574 cDNA clone IMAGE:2487432 ear 5' read
  AI635606 cDNA clone IMAGE:2289163 uterus 3' read 1.1 kbPolyadenylation signal
  BG179854 cDNA clone IMAGE:4430128 adenocarcinoma, cell line 5' read
  BF185691 cDNA clone IMAGE:4041014 glioblastoma 5' read
  AA322973 cDNA clone ATCC:123511 brain 5' read
  AI869755 cDNA clone IMAGE:2434833 uterus 3' read 3.9 kbPolyadenylation signal
  AW473052 cDNA clone IMAGE:2770965 uterus 3' read
  C16496 cDNA clone GEN332B04 aorta 5' read
  AA598567 cDNA clone IMAGE:898195 kidney 3' read 1.9 kb
  AI890730 cDNA clone IMAGE:2443453 uterus 3' read 3.4 kbProtein similarity
  AW161749 cDNA clone IMAGE:2781571 frontal lobe 3' read Protein similarity
  BE349610 cDNA clone IMAGE:3150933 malignant melanoma, metastatic to lymph node 3' read Protein similarity
  BE220919 cDNA clone IMAGE:3170530 malignant melanoma, metastatic to lymph node 3' read
  AU157493 cDNA clone PLACE1007969 placenta 3' read Protein similarityPolyadenylation signal
  AV699659 cDNA clone GKCBUB02 hepatocellular carcinoma 3' read Protein similarity
  AV699696 cDNA clone GKCBUB12 hepatocellular carcinoma 3' read Protein similarity
  AU152831 cDNA clone NT2RP3001792 3' read
  BG484857 cDNA clone IMAGE:4618956 lung 5' read Protein similarity
  BF528254 cDNA clone IMAGE:4180807 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  BE782798 cDNA clone IMAGE:3874941 retinoblastoma 5' read Protein similarity
  BE778844 cDNA clone IMAGE:3869600 retinoblastoma 5' read Protein similarity
  BF240317 cDNA clone IMAGE:4133844 from chronic myelogenous leukemia 5' read Protein similarity
  BG110109 cDNA clone IMAGE:4367431 osteosarcoma, cell line 5' read Protein similarity
  BI871699 cDNA clone IMAGE:5405760 adenocarcinoma, cell line 5' read Protein similarity
  AA360072 cDNA clone ATCC:164201 lung 5' read Protein similarity
  AU138158 cDNA clone PLACE1007969 placenta 5' read Protein similarity
  BG772646 cDNA clone IMAGE:4837673 testis, cell line 5' read Protein similarity
  BG758742 cDNA clone IMAGE:4853257 primary b-cells from tonsils (cell line) 5' read Protein similarity
  AU130981 cDNA clone NT2RP3001792 5' read Protein similarity
  BG718117 cDNA clone IMAGE:4830687 testis, cell line 5' read Protein similarity
  BI829053 cDNA clone IMAGE:5170930 medulla 5' read Protein similarity
  BG719974 cDNA clone IMAGE:4823670 testis, cell line 5' read Protein similarity
  AW161144 cDNA clone IMAGE:2781571 frontal lobe 5' read Protein similarity
  BI561594 cDNA clone IMAGE:5298408 testis, cell line 5' read Protein similarity
  BI458701 cDNA clone IMAGE:5266017 testis, cell line 5' read