Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.47144
Description:DKFZP586N0819 protein
Best-Of-UniGene (BOU) SequenceNM_015437
Genomic Coordiantes Displayed:Bases 258410 to 263671 of contig HsX_11721_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.47144.jpeg
Best sense EST/protein match:NM_015437 matched ref|NP_056252.1| (NM_015437) DKFZP586N0819 protein [Homo sapiens] (E = 1e-49)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BG036452 cDNA clone IMAGE:4428023 adenocarcinoma, cell line 5' read Polyadenylation signal
  AW079750 cDNA clone IMAGE:2585414 moderately differentiated adenocarcinoma 3' read Polyadenylation signal
  AW028954 cDNA clone IMAGE:2542048 stomach 3' read 2.6 kbPolyadenylation signal
  AI824715 cDNA clone IMAGE:2322720 pancreas 3' read 2.5 kbPolyadenylation signal
  AW150151 cDNA clone IMAGE:2629618 uterus 3' read Polyadenylation signal
  AI952267 cDNA clone IMAGE:2547093 two pooled squamous cell carcinomas 3' read 1.5 kbPolyadenylation signal
  AI521422 cDNA clone IMAGE:2122671 ovary 3' read 0.7 kbPolyadenylation signal
  AI566595 cDNA clone IMAGE:2224306 pancreas 3' read 0.9 kbPolyadenylation signal
  AI920793 cDNA clone IMAGE:2452301 uterus 3' read 1.5 kbPolyadenylation signal
  AW051241 cDNA clone IMAGE:2557648 uterus 3' read Polyadenylation signal
  AI689429 cDNA clone IMAGE:2276227 uterus 3' read 2.3 kbPolyadenylation signal
  AI783813 cDNA clone IMAGE:2257251 stomach 3' read Polyadenylation signal
  BG028797 cDNA clone IMAGE:4387873 osteosarcoma, cell line 5' read
  BF106205 cDNA clone IMAGE:4043229 lung 5' read
  AA316516 cDNA clone ATCC:112997 5' read