Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.47313
Description:KIAA0258 gene product
Best-Of-UniGene (BOU) SequenceNM_014785
Genomic Coordiantes Displayed:Bases 1521000 to 1549000 of contig Hs9_28427_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.47313.jpeg
Best sense EST/protein match:NM_014785 matched ref|NP_055600.1| (NM_014785) KIAA0258 gene product [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:BG284168 matched ref|XP_059999.1| (XM_059999) similar to PROSTATE SECRETED SEMINAL PLASMA PROTEIN (E = 3e-82)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI591399 cDNA clone IMAGE:2260122 tumor, 5 pooled (see description) 3' read 0.6 kb
  BF240657 cDNA clone IMAGE:4099778 from acute myelogenous leukemia 5' read
  BG778539 cDNA clone IMAGE:4806617 adenocarcinoma 5' read
  BG178141 cDNA clone IMAGE:4428971 adenocarcinoma, cell line 5' read
  BG036815 cDNA clone IMAGE:4428324 adenocarcinoma, cell line 5' read Polyadenylation signal
  BG284168 cDNA clone IMAGE:4520522 adenocarcinoma, cell line 5' read
  BG498634 cDNA clone IMAGE:4666974 adenocarcinoma 5' read
  BG178634 cDNA clone IMAGE:4429779 adenocarcinoma, cell line 5' read Polyadenylation signal
  BI963718 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  BG779825 cDNA clone IMAGE:4807175 adenocarcinoma 5' read
  BG498362 cDNA clone IMAGE:4666428 adenocarcinoma 5' read
  BF527151 cDNA clone IMAGE:4177637 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BG036362 cDNA clone IMAGE:4427872 adenocarcinoma, cell line 5' read
  BF102551 cDNA clone IMAGE:3931200 adenocarcinoma 5' read
  BG255425 cDNA clone IMAGE:4475899 adenocarcinoma, cell line 5' read
  BF214223 cDNA clone IMAGE:4079183 from acute myelogenous leukemia 5' read
  BG255338 cDNA clone IMAGE:4477974 adenocarcinoma, cell line 5' read
  BI963979 cDNA clone (no-name) islets of langerhans 5' read

SENSE ESTs

  BE263434 cDNA clone IMAGE:3533857 small cell carcinoma 5' read Protein similarity
  BF171997 cDNA clone (no-name) blood Protein similarity
  AW583855 cDNA clone (no-name) islets of langerhans 5' read Protein similarity
  BE019777 cDNA clone IMAGE:3029763 adenocarcinoma cell line 5' read
  BE730177 cDNA clone IMAGE:3833739 melanotic melanoma 5' read
  BE730459 cDNA clone IMAGE:3832549 melanotic melanoma 5' read
  BE730214 cDNA clone IMAGE:3833740 melanotic melanoma 5' read
  BE730538 cDNA clone IMAGE:3831940 melanotic melanoma 5' read
  BG574721 cDNA clone IMAGE:4705525 mammary adenocarcinoma, cell line 5' read
  BM127615 cDNA clone (no-name) islets of langerhans 5' read
  BG912272 cDNA clone IMAGE:4942554 anaplastic oligodendroglioma with 1p/19q loss 5' read
  AI955032 cDNA clone IMAGE:2491068 uterus 3' read 1.6 kb
  BF345954 cDNA clone IMAGE:4153406 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BF345790 cDNA clone IMAGE:4153407 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BG830476 cDNA clone IMAGE:4909089 epithelioid carcinoma cell line 5' read
  AI525216 cDNA clone (no-name) Prostate 5' read
  BF969707 cDNA clone IMAGE:4360392 adrenal cortex carcinoma, cell line 5' read
  AL133780 cDNA clone DKFZp761K2013 amygdala 5' read
  BG025139 cDNA clone IMAGE:4364276 lymphoma, cell line 5' read
  BE890140 cDNA clone IMAGE:3914477 leiomyosarcoma 5' read
  BE548610 cDNA clone IMAGE:3459812 choriocarcinoma 5' read
  BE733466 cDNA clone IMAGE:3843711 choriocarcinoma 5' read Protein similarity
  BE407736 cDNA clone IMAGE:3629540 choriocarcinoma 5' read
  BE731145 cDNA clone IMAGE:3841295 choriocarcinoma 5' read
  BE783125 cDNA clone IMAGE:3873775 retinoblastoma 5' read
  BE546604 cDNA clone IMAGE:3461576 choriocarcinoma 5' read
  D31490 cDNA clone (no-name) lung 5' read
  BG420557 cDNA clone IMAGE:4586885 renal cell adenocarcinoma 5' read
  BE396062 cDNA clone IMAGE:3659852 endometrium, adenocarcinoma cell line 5' read
  AW440541 cDNA clone IMAGE:2889597 uterus 3' read
  AW173479 cDNA clone IMAGE:2656538 uterus 3' read
  AA341631 cDNA clone ATCC:143447 kidney 5' read
  AA373732 cDNA clone ATCC:178345 5' read
  AI953828 cDNA clone IMAGE:2548968 three pooled meningiomas 3' read 1.4 kb
  AI061125 cDNA clone IMAGE:1700667 kidney 3' read Polyadenylation signal
  BF679930 cDNA clone IMAGE:4295622 prostate 5' read
  R45884 cDNA clone CBS313 eye 5' read
  F15747 cDNA clone NOTAVAIL00217
  BG704102 cDNA clone IMAGE:4819697 hippocampus, cell line 5' read
  BF218096 cDNA clone IMAGE:4095454 glioblastoma 5' read