Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.48650
Description:hypothetical protein MGC16186
Best-Of-UniGene (BOU) SequenceNM_032372
Genomic Coordiantes Displayed:Bases 482000 to 538000 of contig Hs10_19664_24
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingHs.48650.jpeg
Best sense EST/protein match:NM_032372 matched ref|NP_115748.1| (NM_032372) hypothetical protein MGC16186 [Homo sapiens] (E = e-100)
Best antisense EST/protein match:BI828082 matched gb|AAH19250.1|AAH19250 (BC019250) Unknown (protein for MGC:26601) [Homo sapiens] (E = 4e-74)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG718556 cDNA clone IMAGE:4828878 testis, cell line 5' read Protein similarity
  BI828082 cDNA clone IMAGE:5167113 medulla 5' read
  BI546816 cDNA clone IMAGE:5261306 hippocampus, cell line 5' read Protein similarity
  BI559804 cDNA clone IMAGE:5294928 testis, cell line 5' read Protein similarity
  BI829226 cDNA clone IMAGE:5171266 medulla 5' read
  AL040530 cDNA clone DKFZp434H1714 testis 5' read

SENSE ESTs

  BE408275 cDNA clone IMAGE:3637472 choriocarcinoma 5' read
  BE385286 cDNA clone IMAGE:3618661 melanotic melanoma 5' read
  D81849 cDNA clone GEN414E07 brain 5' read
  BE894515 cDNA clone IMAGE:3918509 melanotic melanoma 5' read