Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.5400
Description:KIAA1018 protein
Best-Of-UniGene (BOU) SequenceNM_014967
Genomic Coordiantes Displayed:Bases 1290000 to 1360000 of contig Hs15_10520_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.5400.jpeg
Best sense EST/protein match:BE873144 matched ref|XP_007768.3| (XM_007768) KIAA1018 protein [Homo sapiens] (E = e-100)
Best antisense EST/protein match:AA001921 matched ref|XP_058715.1| (XM_058715) similar to KIAA1682 protein (H. sapiens) [Homo sapiens] (E = 2e-46)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG281593 cDNA clone IMAGE:4544843 melanotic melanoma 5' read
  BE542036 cDNA clone IMAGE:3453346 placenta 5' read
  AA001945 cDNA clone IMAGE:427882 pool 3' read 0.8 kbProtein similarity
  AA001921 cDNA clone IMAGE:427906 pool 3' read 0.8 kbProtein similarity
  BG751186 cDNA clone IMAGE:4873532 normal pigmented retinal epithelium 5' read
  BG475282 cDNA clone IMAGE:4620465 melanotic melanoma 5' read
  BG747926 cDNA clone IMAGE:4842450 normal pigmented retinal epithelium 5' read
  BE387704 cDNA clone IMAGE:3621566 endometrium, adenocarcinoma cell line 5' read
  BE407221 cDNA clone IMAGE:3635720 choriocarcinoma 5' read Protein similarity
  BE383948 cDNA clone IMAGE:3614256 melanotic melanoma 5' read Protein similarity
  AA001140 cDNA clone IMAGE:427882 pool 5' read 0.8 kb
  AA001284 cDNA clone IMAGE:427906 pool 5' read 0.8 kb
  AA093869 cDNA clone (no-name) heart 5' read

SENSE ESTs

  BI767179 cDNA clone IMAGE:5209981 pooled lung and spleen 5' read
  BG542552 cDNA clone IMAGE:4696285 lung 5' read
  BF034978 cDNA clone IMAGE:3862786 adenocarcinoma 5' read
  BE873144 cDNA clone IMAGE:3855565 adenocarcinoma 5' read Protein similarity
  BF968118 cDNA clone IMAGE:4357462 adrenal cortex carcinoma, cell line 5' read
  BI850370 cDNA clone IMAGE:4357462 adrenal cortex carcinoma, cell line 5' read Protein similarity
  BF572780 cDNA clone IMAGE:4243757 melanotic melanoma, high mdr 5' read
  BG574314 cDNA clone IMAGE:4705374 mammary adenocarcinoma, cell line 5' read
  BG470967 cDNA clone IMAGE:4634411 retinoblastoma 5' read Protein similarity
  BG329239 cDNA clone IMAGE:4558057 retinoblastoma 5' read
  BG395775 cDNA clone IMAGE:4581130 retinoblastoma 5' read
  AW663181 cDNA clone IMAGE:2968643 2 pooled high-grade transitional cell tumors 5' read Protein similarity
  BI829682 cDNA clone IMAGE:5171575 medulla 5' read Protein similarity
  AL601320 cDNA clone DKFZp313O1440 5' read Protein similarity
  BG681642 cDNA clone IMAGE:4752899 squamous cell carcinoma 5' read Protein similarity
  AA323109 cDNA clone ATCC:123637 brain 5' read Protein similarity
  F11513 cDNA clone c2ya01 brain
  AA329835 cDNA clone ATCC:131494 whole embryo 5' read
  AA337726 cDNA clone ATCC:139640 uterus 5' read Protein similarity
  BI964799 cDNA clone (no-name) islets of langerhans 5' read
  BG677682 cDNA clone IMAGE:4750186 squamous cell carcinoma 5' read
  R25547 cDNA clone IMAGE:36597 brain 5' read 2.4 kbProtein similarity
  BG687320 cDNA clone IMAGE:4762768 mucoepidermoid carcinoma 5' read
  AA383430 cDNA clone ATCC:187764 testis 5' read
  BF342858 cDNA clone IMAGE:4150714 glioblastoma with egfr amplification 5' read Polyadenylation signal
  AI859495 cDNA clone IMAGE:2435752 uterus 3' read 0.7 kbPolyadenylation signal
  AW151811 cDNA clone IMAGE:2623343 stomach 3' read Polyadenylation signal
  AI344569 cDNA clone IMAGE:1917486 kidney 3' read 1.2 kbPolyadenylation signal
  AI564450 cDNA clone IMAGE:2212915 uterus 3' read Polyadenylation signal
  AW027817 cDNA clone IMAGE:2533962 stomach 3' read Polyadenylation signal
  AW613627 cDNA clone IMAGE:2968643 2 pooled high-grade transitional cell tumors 3' read Polyadenylation signal
  AW073514 cDNA clone IMAGE:2568932 five pooled sarcomas, including myxoid liposarcoma, solitary 3' read Polyadenylation signal
  R46703 cDNA clone IMAGE:36597 brain 3' read 2.4 kbPolyadenylation signal
  AW511773 cDNA clone IMAGE:2807672 kidney 3' read Polyadenylation signal
  F09174 cDNA clone c2ya01 brain 3' read
  BM023522 cDNA clone (no-name) islets of langerhans 5' read Polyadenylation signal
  BM023233 cDNA clone (no-name) islets of langerhans 3' read Polyadenylation signal
  AI680065 cDNA clone IMAGE:2264483 uterus 3' read 1.9 kbPolyadenylation signal