Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.55923
Description:hypothetical protein MGC4655
Best-Of-UniGene (BOU) SequenceNM_033309
Genomic Coordiantes Displayed:Bases 1584000 to 1696000 of contig Hs16_10635_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.55923.jpeg
Best sense EST/protein match:BI196765 matched ref|NP_171608.1| (NM_033309) hypothetical protein MGC4655 [Homo sapiens] (E = e-119)
Best antisense EST/protein match:BG482194 matched gb|AAH04556.1|AAH04556 (BC004556) Similar to CG7083 gene product [Homo sapiens] (E = e-109)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AI689566 cDNA clone IMAGE:2276265 uterus 3' read 1.6 kbPolyadenylation signal
  AW082824 cDNA clone IMAGE:2583194 moderately differentiated adenocarcinoma 3' read
  BG825219 cDNA clone IMAGE:4900496 rhabdomyosarcoma 5' read
  AI640864 cDNA clone IMAGE:2293844 pancreas 3' read Polyadenylation signal
  AV746318 cDNA clone NPAAMF09 pituitary 5' read
  W47500 cDNA clone IMAGE:324211 3' read 2.6 kb
  AV745361 cDNA clone NPAAAB02 pituitary 5' read
  AI023696 cDNA clone IMAGE:1654211 pool 3' read 0.6 kbPolyadenylation signal
  AI453251 cDNA clone IMAGE:2143947 pancreas 3' read 2.4 kbPolyadenylation signal
  Z41775 cDNA clone c1cb03 brain 3' read Polyadenylation signal
  BG491823 cDNA clone IMAGE:4684944 amelanotic melanoma, cell line 3' read
  BI252715 cDNA clone IMAGE:5086911 hepatocellular carcinoma, cell line 3' read
  BG491581 cDNA clone IMAGE:4684944 amelanotic melanoma, cell line 5' read
  BF346949 cDNA clone IMAGE:4157231 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BF032181 cDNA clone IMAGE:3828528 hypernephroma 5' read Protein similarity
  AW957426 cDNA clone (no-name)
  BG697911 cDNA clone IMAGE:4804234 skin 5' read
  BI908669 cDNA clone IMAGE:5216014 leukocyte 5' read Protein similarity
  AA377725 cDNA clone ATCC:182123 synovial membrane 5' read
  BF343440 cDNA clone IMAGE:4150405 glioblastoma with egfr amplification 5' read Protein similarity
  BE547306 cDNA clone IMAGE:3459872 choriocarcinoma 5' read Protein similarity
  AA326810 cDNA clone ATCC:127476 brain 5' read Protein similarity
  BF036229 cDNA clone IMAGE:3862564 adenocarcinoma 5' read Protein similarity
  BI252029 cDNA clone IMAGE:5086939 hepatocellular carcinoma, cell line 5' read Protein similarity
  BI916445 cDNA clone IMAGE:5246473 brain 5' read