Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.82143
Description:E74-like factor 2 (ets domain transcription factor)
Best-Of-UniGene (BOU) SequenceNM_006874
Genomic Coordiantes Displayed:Bases 949167 to 1069282 of contig Hs4_6281_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.82143.jpeg
Best sense EST/protein match:BI770157 matched gb|AAB37760.1| (U43189) NERF-1a [Homo sapiens] (E = e-110)
Best antisense EST/protein match:BM021141 matched dbj|BAB30183.1| (AK016297) evidence:NAS~hypothetical protein~putative [Mus (E = 1e-40)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI966045 cDNA clone (no-name) islets of langerhans 3' read
  BF198002 cDNA clone IMAGE:3653082 skin, normal, 4 pooled samples 3' read
  BM021141 cDNA clone (no-name) islets of langerhans 3' read
  BI966233 cDNA clone (no-name) islets of langerhans 5' read
  BM021401 cDNA clone (no-name) islets of langerhans 5' read
  AI049965 cDNA clone IMAGE:1700957 kidney 3' read
  AI061117 cDNA clone IMAGE:1699795 kidney 3' read

SENSE ESTs

  BI906453 cDNA clone IMAGE:5212532 leukocyte 5' read Protein similarity
  BI914702 cDNA clone IMAGE:5243411 brain 5' read
  BG717146 cDNA clone IMAGE:4821608 testis, cell line 5' read Protein similarity
  BI460176 cDNA clone IMAGE:5267848 testis, cell line 5' read
  BI770157 cDNA clone IMAGE:5202783 pooled lung and spleen 5' read Protein similarity
  BG897412 cDNA clone (no-name) cartilage Protein similarity
  AL121305 cDNA clone DKFZp762M166 melanoma (mewo cell line) 5' read Protein similarity
  BI820226 cDNA clone IMAGE:5177824 pooled brain, lung, testis 5' read Protein similarity
  BF969789 cDNA clone IMAGE:4360495 adrenal cortex carcinoma, cell line 5' read Protein similarity
  BG258152 cDNA clone IMAGE:4510068 embryonal carcinoma, cell line 5' read Protein similarity
  BI767286 cDNA clone IMAGE:5207069 pooled lung and spleen 5' read Protein similarity
  BG259512 cDNA clone IMAGE:4509461 embryonal carcinoma, cell line 5' read Protein similarity
  AA247414 cDNA clone (no-name) heart 5' read Protein similarity
  AA112815 cDNA clone IMAGE:561795 muscle 5' read Protein similarity
  BE620913 cDNA clone IMAGE:3886050 large cell carcinoma, undifferentiated 3' read Protein similarity
  BE620515 cDNA clone IMAGE:3886050 large cell carcinoma, undifferentiated 5' read Protein similarity
  R12546 cDNA clone IMAGE:26130 brain 5' read 1.3 kbProtein similarity
  AV682364 cDNA clone GKBAMC01 hepatocellular carcinoma 5' read
  AI632360 cDNA clone IMAGE:2181968 uterus 3' read 1.4 kbPolyadenylation signal
  AI241722 cDNA clone IMAGE:1977155 brain 3' read 1.1 kbPolyadenylation signal
  BG108004 cDNA clone IMAGE:4367692 osteosarcoma, cell line 5' read
  AA086254 cDNA clone IMAGE:561795 muscle 3' read
  AI921450 cDNA clone IMAGE:2456346 stomach 3' read 0.5 kb
  AW150755 cDNA clone IMAGE:2629880 uterus 3' read
  AW151632 cDNA clone IMAGE:2623086 stomach 3' read
  AI344581 cDNA clone IMAGE:1917495 kidney 3' read 1.1 kb
  AI347752 cDNA clone IMAGE:1916849 kidney 3' read 2.2 kb
  R37447 cDNA clone IMAGE:26130 brain 3' read 1.3 kb
  AI536582 cDNA clone IMAGE:2184715 stomach 3' read