Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.85339
Description:G protein-coupled receptor 39
Best-Of-UniGene (BOU) SequenceNM_001508
Genomic Coordiantes Displayed:Bases 492000 to 548000 of contig Hs2_5168_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.85339.jpeg
Best sense EST/protein match:NM_001508 matched ref|NP_001499.1| (NM_001508) G protein-coupled receptor 39 [Homo sapiens] (E = 0.0)
Best antisense EST/protein match:BI917149 matched ref|XP_059408.1| (XM_059408) similar to unnamed protein product (H. sapiens) [Homo (E = 2e-69)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW148557 cDNA clone IMAGE:2616621 brain 3' read Protein similarity
  AW128849 cDNA clone IMAGE:2596847 ovary 3' read Protein similarity
  BF219218 cDNA clone IMAGE:4094584 glioblastoma 5' read Protein similarity
  BE899287 cDNA clone IMAGE:3952206 adenocarcinoma cell line 5' read
  AI859538 cDNA clone IMAGE:2435812 uterus 3' read 1.9 kb
  BF726459 cDNA clone by06h03 lens 5' read Protein similarity
  AW150789 cDNA clone IMAGE:2629922 uterus 3' read Protein similarity
  AW075549 cDNA clone IMAGE:2577056 2 pooled wilms' tumors, one primary and one metastatic to 3' read Protein similarity
  AW087372 cDNA clone IMAGE:2576786 2 pooled wilms' tumors, one primary and one metastatic to 3' read Protein similarity
  AW191974 cDNA clone IMAGE:2680778 pancreas 3' read Protein similarity
  H17965 cDNA clone IMAGE:50205 brain 3' read 1.6 kb
  R17696 cDNA clone IMAGE:25506 brain 3' read 1.4 kb
  H06707 cDNA clone IMAGE:44619 brain 3' read 1.7 kb
  R43737 cDNA clone IMAGE:32715 brain 3' read 1.6 kb
  AW075598 cDNA clone IMAGE:2577252 2 pooled wilms' tumors, one primary and one metastatic to 3' read Protein similarity
  AW338938 cDNA clone IMAGE:2878903 pancreas 3' read Protein similarity
  AW771806 cDNA clone IMAGE:3006754 2 pooled wilms' tumors, one primary and one metastatic to 3' read
  AW768971 cDNA clone IMAGE:3006256 2 pooled wilms' tumors, one primary and one metastatic to 3' read
  AA084249 cDNA clone IMAGE:547721 3' read 1.0 kbProtein similarity
  AA665640 cDNA clone IMAGE:1126871 kidney 3' read Protein similarity
  BF125134 cDNA clone IMAGE:4025340 melanotic melanoma 5' read Protein similarity
  BF126050 cDNA clone IMAGE:4026092 melanotic melanoma 5' read Protein similarity
  BE385990 cDNA clone IMAGE:3617696 melanotic melanoma 5' read Protein similarity
  AI499630 cDNA clone IMAGE:2177941 uterus 3' read 0.8 kbProtein similarityPolyadenylation signal
  R41205 cDNA clone IMAGE:29218 brain 3' read 1.4 kbProtein similarityPolyadenylation signal
  AA496806 cDNA clone IMAGE:897659 kidney 3' read 1.5 kbProtein similarityPolyadenylation signal
  BF726644 cDNA clone by09g04 lens 5' read Protein similarity
  BE350014 cDNA clone IMAGE:3146086 2 pooled wilms' tumors, one primary and one metastatic to 3' read Protein similarity
  BG752229 cDNA clone IMAGE:4875113 normal pigmented retinal epithelium 5' read Protein similarity
  BE395581 cDNA clone IMAGE:3631256 endometrium, adenocarcinoma cell line 5' read Protein similarity
  BE395206 cDNA clone IMAGE:3631047 endometrium, adenocarcinoma cell line 5' read Protein similarity
  BE378674 cDNA clone IMAGE:3609308 endometrium, adenocarcinoma cell line 5' read Protein similarity
  AA084248 cDNA clone IMAGE:547721 5' read 1.0 kbProtein similarity
  BE395797 cDNA clone IMAGE:3631543 endometrium, adenocarcinoma cell line 5' read Protein similarity
  BE271770 cDNA clone IMAGE:3141450 adenocarcinoma cell line 5' read
  R11723 cDNA clone IMAGE:25506 brain 5' read 1.4 kb
  BE392412 cDNA clone IMAGE:3627134 endometrium, adenocarcinoma cell line 5' read
  AV725028 cDNA clone HTCBRH07 hypothalamus 5' read
  BI831919 cDNA clone IMAGE:5170606 medulla 5' read
  R14544 cDNA clone IMAGE:29218 brain 5' read 1.4 kb
  BG753617 cDNA clone IMAGE:4876392 normal pigmented retinal epithelium 5' read
  R20352 cDNA clone IMAGE:32715 brain 5' read 1.6 kb
  H17189 cDNA clone IMAGE:50205 brain 5' read 1.6 kb
  BF345141 cDNA clone IMAGE:4153684 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BG753353 cDNA clone IMAGE:4875499 normal pigmented retinal epithelium 5' read
  BI823714 cDNA clone IMAGE:5181585 pooled brain, lung, testis 5' read
  BI554034 cDNA clone IMAGE:5309527 anaplastic oligodendroglioma with 1p/19q loss 5' read
  BI917149 cDNA clone IMAGE:5245648 brain 5' read
  BF125664 cDNA clone IMAGE:4026320 melanotic melanoma 5' read
  BI913989 cDNA clone IMAGE:5244569 brain 5' read
  BE736301 cDNA clone IMAGE:3641660 adenocarcinoma 5' read
  BF125425 cDNA clone IMAGE:4026252 melanotic melanoma 5' read
  BE746601 cDNA clone IMAGE:3928868 adenocarcinoma cell line 5' read
  BI669845 cDNA clone IMAGE:5312889 hypothalamus, cell line 5' read
  BE386060 cDNA clone IMAGE:3617749 melanotic melanoma 5' read
  H06756 cDNA clone IMAGE:44619 brain 5' read 1.7 kb
  BG704350 cDNA clone IMAGE:4819871 hippocampus, cell line 5' read
  AA102212 cDNA clone IMAGE:525929 5' read 1.3 kb
  BI913002 cDNA clone IMAGE:5240715 brain 5' read