Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.94037
Description:hypothetical protein FLJ23053
Best-Of-UniGene (BOU) SequenceNM_022907
Genomic Coordiantes Displayed:Bases 188400 to 199600 of contig Hs5_7011_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.94037.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:AA328639 matched ref|XP_028551.1| (XM_028551) hypothetical protein XP_028551 [Homo sapiens] (E = 5e-13)

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  AI682757 cDNA clone IMAGE:2323539 pancreas 3' read 4.2 kbPolyadenylation signal
  AL600062 cDNA clone DKFZp313A0232 5' read
  BF690055 cDNA clone IMAGE:4298390 melanotic melanoma, high mdr (cell line) 3' read Protein similarity
  AA205660 cDNA clone IMAGE:646814 3' read Polyadenylation signal
  D81272 cDNA clone GEN142G09 brain 5' read
  AA328639 cDNA clone ATCC:130289 whole embryo 5' read
  BE543847 cDNA clone IMAGE:3457735 choriocarcinoma 5' read Protein similarity
  AI926915 cDNA clone IMAGE:2444139 uterus 3' read 0.9 kb
  AW673910 cDNA clone IMAGE:2985685 choriocarcinoma 3' read
  AI570340 cDNA clone IMAGE:2184382 stomach 3' read 1.4 kb
  AI358593 cDNA clone IMAGE:1993574 uterus 3' read 1.2 kb
  AW440430 cDNA clone IMAGE:2890483 uterus 3' read
  BF344579 cDNA clone IMAGE:4150847 glioblastoma with egfr amplification 5' read
  AL597542 cDNA clone DKFZp313H2114 5' read
  BG819055 cDNA clone IMAGE:4932126 anaplastic oligodendroglioma with 1p/19q loss 5' read Protein similarity
  AA360987 cDNA clone ATCC:165132 lymph 5' read


  BG033726 cDNA clone IMAGE:4402534 mammary adenocarcinoma, cell line 5' read