Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Hs.94769
Description:RAB23, member RAS oncogene family
Best-Of-UniGene (BOU) SequenceAF161486
Genomic Coordiantes Displayed:Bases 1488215 to 1535111 of contig Hs6_23575_24
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingHs.94769.jpeg
Best sense EST/protein match:AF161486 matched gb|AAF29101.1|AF161486_1 (AF161486) HSPC137 [Homo sapiens] (E = e-132)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI619610 cDNA clone IMAGE:2282562 uterus 3' read 0.3 kbProtein similarityPolyadenylation signal
  BG501764 cDNA clone IMAGE:4654905 embryonal carcinoma 5' read
  AI078466 cDNA clone IMAGE:1675240 pool 3' read 0.7 kbPolyadenylation signal
  AA705004 cDNA clone IMAGE:462662 pool 3' read Polyadenylation signal
  AA694297 cDNA clone IMAGE:432781 pool 3' read Polyadenylation signal
  AA704987 cDNA clone IMAGE:462629 pool 3' read Polyadenylation signal
  AA779313 cDNA clone IMAGE:454029 pool 3' read

SENSE ESTs

  BE890519 cDNA clone IMAGE:3916751 melanotic melanoma 5' read Protein similarity
  R35591 cDNA clone IMAGE:38191 brain 5' read 1.5 kb
  AV682098 cDNA clone GKBAOEO4 hepatocellular carcinoma 5' read
  D79953 cDNA clone GEN362H04 aorta 5' read
  AV738036 cDNA clone CBFBDH05 cord blood 5' read Polyadenylation signal
  D62821 cDNA clone GEN329C09 aorta 5' read
  AI628941 cDNA clone IMAGE:2281570 uterus 3' read 0.8 kbPolyadenylation signal
  AI951271 cDNA clone IMAGE:2548559 four pooled high-grade tumors, including two primary tumors 3' read Polyadenylation signal
  AW515369 cDNA clone IMAGE:2809515 uterus 3' read Polyadenylation signal
  AI624225 cDNA clone IMAGE:2229872 pancreas 3' read 2.4 kbPolyadenylation signal
  R37178 cDNA clone IMAGE:27144 brain 3' read 1.6 kbPolyadenylation signal
  AA705300 cDNA clone IMAGE:462832 pool 3' read
  AI537589 cDNA clone IMAGE:2186864 stomach 3' read 2.4 kbPolyadenylation signal
  T23992 cDNA clone 3hflsk2081 pool 3' read
  D61963 cDNA clone GEN227B09 aorta 5' read
  AW571955 cDNA clone IMAGE:2840151 uterus 3' read Polyadenylation signal
  D79490 cDNA clone GEN256H01 aorta 5' read
  D79493 cDNA clone GEN257D05 aorta 5' read
  D61830 cDNA clone GEN214A08 aorta 5' read
  D61796 cDNA clone GEN212A06 aorta 5' read
  D61863 cDNA clone GEN218B07 aorta 5' read
  D79302 cDNA clone GEN221D06 aorta 5' read
  D79395 cDNA clone GEN238G04 aorta 5' read
  T16460 cDNA clone NIB1338 brain 3' read Polyadenylation signal
  AI699282 cDNA clone IMAGE:2323249 pancreas 3' read 3.1 kbPolyadenylation signal