Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.10022
Description:homer, neuronal immediate early gene, 3
Best-Of-UniGene (BOU) SequenceBC005773
Genomic Coordiantes Displayed:Bases 655515 to 682791 of contig GA_x5J8B7W3T6H
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.10022.jpeg
Best sense EST/protein match:BC005773 matched gb|AAH05773.1|AAH05773 (BC005773) Similar to Homer, neuronal immediate early gene, 3 [Mus (E = e-166)
Best antisense EST/protein match:BG962581 matched gb|AAB81544.1| (AC002985) R27090_2 [Homo sapiens] (E = e-104)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF539224 cDNA clone IMAGE:4192389 salivary gland 5' read Protein similarity
  AA544796 cDNA clone IMAGE:948613 mammary gland 5' read Protein similarity
  AI647511 cDNA clone IMAGE:1971502 kidney 3' read Protein similarityPolyadenylation signal
  AI561848 cDNA clone IMAGE:948613 mammary gland 5' read Protein similarity
  AI505082 cDNA clone IMAGE:948613 mammary gland 3' read Protein similarity
  AA771156 cDNA clone IMAGE:1163444 muscle 5' read
  BF133631 cDNA clone IMAGE:4006829 tumor, metastatic to mammary 5' read Protein similarity
  AI327089 cDNA clone IMAGE:422802 embryo 3' read Protein similarity
  AI662691 cDNA clone IMAGE:737945 liver 3' read Protein similarity
  AI116264 cDNA clone IMAGE:1398211 mammary gland 5' read Protein similarity
  AI325878 cDNA clone IMAGE:422802 embryo 5' read Protein similarity
  AI021635 cDNA clone IMAGE:1366893 mammary gland 5' read Protein similarity
  BI455349 cDNA clone IMAGE:5250799 tumor, gross tissue 5' read
  BF140299 cDNA clone IMAGE:4015633 tumor, metastatic to mammary 5' read Protein similarity
  BF786436 cDNA clone IMAGE:4240960 kidney 5' read Protein similarity
  BF786352 cDNA clone IMAGE:4240961 kidney 5' read Protein similarity
  BG962581 cDNA clone IMAGE:4984934 colon 5' read Protein similarity
  BI872367 cDNA clone IMAGE:5400586 retina 5' read Protein similarity
  BF016321 cDNA clone IMAGE:3662158 tumor, metastatic to mammary 5' read Protein similarity
  AL034707 cDNA clone 528_8L22 dissected endoderm 5' read Protein similarity
  AW258678 cDNA clone IMAGE:2811713 tumor, gross tissue 5' read Protein similarity
  AA271508 cDNA clone IMAGE:737945 liver 5' read Protein similarity
  AI006137 cDNA clone IMAGE:1364433 mammary gland 5' read Protein similarity
  AI509061 cDNA clone IMAGE:737945 liver 5' read Protein similarity

SENSE ESTs

  BF383197 cDNA clone IMAGE:4194508 liver 5' read Protein similarity
  BI666229 cDNA clone IMAGE:5324984 infiltrating ductal carcinoma 5' read Protein similarity
  BG976283 cDNA clone IMAGE:4976989 infiltrating ductal carcinoma 5' read Protein similarity
  BE569374 cDNA clone IMAGE:3602414 infiltrating ductal carcinoma 5' read Protein similarity
  BF124419 cDNA clone IMAGE:4024499 tumor, gross tissue 5' read Protein similarity
  BF100180 cDNA clone IMAGE:3979980 tumor, biopsy sample 5' read Protein similarity
  AA770784 cDNA clone IMAGE:1162828 muscle 5' read Protein similarity
  AI592471 cDNA clone IMAGE:1162828 muscle 5' read Protein similarity
  BI559055 cDNA clone IMAGE:5294214 tumor, gross tissue 5' read
  BI659283 cDNA clone IMAGE:5347816 tumor, gross tissue 5' read
  BE372418 cDNA clone IMAGE:3582493 tumor, biopsy sample 5' read
  BE282560 cDNA clone IMAGE:3494547 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI156686 cDNA clone IMAGE:5062225 tumor, gross tissue 5' read Protein similarity
  BG917594 cDNA clone IMAGE:4949353 infiltrating ductal carcinoma 5' read
  BI557308 cDNA clone IMAGE:5291234 tumor, gross tissue 5' read
  AA241382 cDNA clone IMAGE:670938 embryo 5' read
  BG806530 cDNA clone (no-name) neural retina
  BI691744 cDNA clone IMAGE:5343421 infiltrating ductal carcinoma 5' read
  BG298428 cDNA clone IMAGE:4512040 retina 5' read
  AI591447 cDNA clone IMAGE:1162828 muscle 3' read
  AI466720 cDNA clone IMAGE:670721 embryo 5' read
  AA265663 cDNA clone IMAGE:670721 embryo 5' read
  AI666805 cDNA clone IMAGE:670721 embryo 3' read Polyadenylation signal
  BI106797 cDNA clone IMAGE:5035714 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity