Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.10167
Description:RIKEN cDNA 1110055A02 gene
Best-Of-UniGene (BOU) SequenceBC007156
Genomic Coordiantes Displayed:Bases 1711138 to 1756130 of contig GA_x5J8B7W4JVK
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.10167.jpeg
Best sense EST/protein match:BC007156 matched dbj|BAB31305.1| (AK018609) data source:SPTR, source key:Q15649, evidence:ISS~homolog (E = 0.0)
Best antisense EST/protein match:BG088775 matched dbj|BAB22434.1| (AK002888) data source:SPTR, source key:Q15649, (E = 3e-83)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW541320 cDNA clone C0143G05 3' read Polyadenylation signal
  AW549804 cDNA clone L0055D10 3' read Protein similarityPolyadenylation signal
  BI715063 cDNA clone (no-name) 3' read Protein similarityPolyadenylation signal
  AU019090 cDNA clone J0512G10 embryo 3' read Polyadenylation signal
  AU042243 cDNA clone J1015H09 3' read Polyadenylation signal
  AU020892 cDNA clone J0539F01 embryo 3' read Polyadenylation signal
  AU019132 cDNA clone J0513C10 embryo 3' read Polyadenylation signal
  BI453986 cDNA clone IMAGE:5253404 tumor, gross tissue 5' read Protein similarity
  BF781080 cDNA clone IMAGE:4235213 kidney 5' read Protein similarity
  BG076250 cDNA clone H3157D09 3' read Protein similarity
  AW558460 cDNA clone L0296F11 3' read Protein similarity
  BG861609 cDNA clone IMAGE:4916325 tumor, gross tissue 5' read Protein similarity
  W99082 cDNA clone IMAGE:421482 embryo 5' read Protein similarity
  BG861623 cDNA clone IMAGE:4916233 tumor, gross tissue 5' read Protein similarity
  BG088775 cDNA clone H3157D09 5' read Protein similarity
  AA688753 cDNA clone IMAGE:1111083 embryo 5' read Protein similarity
  AA967628 cDNA clone IMAGE:1616715 hypothalamus 5' read Protein similarity
  AI118742 cDNA clone IMAGE:1397831 mammary gland 5' read Protein similarity
  AI153158 cDNA clone IMAGE:1449839 uterus 5' read Protein similarity
  W83288 cDNA clone IMAGE:406141 embryo 5' read Protein similarity
  AA155350 cDNA clone IMAGE:540692 embryo 5' read Protein similarity
  W65952 cDNA clone IMAGE:386890 embryo 5' read Protein similarity
  AA560335 cDNA clone IMAGE:977323 embryo 5' read Protein similarity
  AA967974 cDNA clone IMAGE:1617072 hypothalamus 5' read Protein similarity
  BB564457 cDNA clone 2010014G03 small intestine 5' read Protein similarity
  BB599004 cDNA clone C820003J08 pancreas 5' read Protein similarity

SENSE ESTs

  BI454977 cDNA clone IMAGE:5252748 tumor, gross tissue 5' read Protein similarity
  BE310073 cDNA clone IMAGE:3486558 tumor, gross tissue 5' read
  BI647647 cDNA clone IMAGE:5319204 tumor, gross tissue 5' read
  BI156821 cDNA clone IMAGE:5061564 tumor, gross tissue 5' read
  BI105588 cDNA clone IMAGE:5036864 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AA396567 cDNA clone IMAGE:570359 embryo 5' read
  BG069847 cDNA clone H3080G06 3' read Polyadenylation signal
  AU020932 cDNA clone J0540C07 embryo 3' read Polyadenylation signal
  BF148894 cDNA clone IMAGE:3662817 tumor, metastatic to mammary 3' read Polyadenylation signal