Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.12915
Description:nitrilase 1
Best-Of-UniGene (BOU) SequenceAK004988
Genomic Coordiantes Displayed:Bases 232000 to 246000 of contig GA_x5J8B7W3TEM
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.12915.jpeg
Best sense EST/protein match:BF385518 matched gb|AAC40184.1| (AF069985) nitrilase homolog 1 [Mus musculus] (E = e-116)
Best antisense EST/protein match:BE199280 matched gb|AAD16415.1| (AF100342) death effector domain-containing protein DEDPRO1 [Mus (E = 1e-11)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA986460 cDNA clone IMAGE:1480254 embryo 3' read Polyadenylation signal
  AA137971 cDNA clone IMAGE:595875 spleen 5' read 0.2 kb
  AW228136 cDNA clone IMAGE:2654268 tumor, gross tissue 3' read Polyadenylation signal
  BE447532 cDNA clone IMAGE:3330984 spleen 3' read Polyadenylation signal
  BI688903 cDNA clone IMAGE:5344597 infiltrating ductal carcinoma 5' read
  AA271030 cDNA clone IMAGE:737976 liver 5' read
  W54454 cDNA clone IMAGE:367774 embryo 5' read
  W71253 cDNA clone IMAGE:389343 embryo 5' read
  W71869 cDNA clone IMAGE:390567 embryo 5' read
  AA614938 cDNA clone IMAGE:1051582 colon 5' read
  AA590974 cDNA clone IMAGE:1025652 colon 5' read
  BE199280 cDNA clone IMAGE:3256319 tumor, gross tissue 3' read

SENSE ESTs

  BB622502 cDNA clone 6430409G12 olfactory brain 5' read
  BF385518 cDNA clone IMAGE:4196559 liver 5' read Protein similarity
  AA272231 cDNA clone IMAGE:761706 pool 5' read Protein similarity
  BI694973 cDNA clone IMAGE:5374024 tumor, biopsy sample 5' read
  BI327600 cDNA clone IMAGE:5132565 liver 5' read Protein similarity
  AA041757 cDNA clone IMAGE:475072 embryo 5' read Protein similarity
  AA423741 cDNA clone IMAGE:832282 mammary gland 5' read
  AA212962 cDNA clone IMAGE:676896 liver 5' read Protein similarity
  AA672034 cDNA clone IMAGE:963142 mammary gland 5' read Protein similarity
  BI247226 cDNA clone IMAGE:5126342 liver 5' read
  BE652395 cDNA clone UIM-AO0-acc-a-08-0-UI- 5' read Protein similarity
  AA212834 cDNA clone IMAGE:677357 liver 5' read
  BF780924 cDNA clone IMAGE:4223648 kidney 5' read Protein similarity
  BG294701 cDNA clone IMAGE:4504120 retina 5' read Protein similarity
  BG963013 cDNA clone IMAGE:4982813 colon 5' read Protein similarity
  BI100771 cDNA clone IMAGE:5041264 kidney 5' read Protein similarity
  BF780847 cDNA clone IMAGE:4223624 kidney 5' read Protein similarity
  BI157838 cDNA clone IMAGE:5063422 tumor, gross tissue 5' read
  W41887 cDNA clone IMAGE:353286 embryo 5' read
  BI647584 cDNA clone IMAGE:5318631 tumor, gross tissue 5' read Protein similarity
  AI785847 cDNA clone IMAGE:1888679 liver 5' read Protein similarity
  BI332807 cDNA clone IMAGE:5138032 liver 5' read Protein similarity
  BE226960 cDNA clone IMAGE:3256965 tumor, gross tissue 5' read
  AW319331 cDNA clone IMAGE:2395050 kidney 5' read Protein similarity
  BF168490 cDNA clone IMAGE:4017740 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BE306755 cDNA clone IMAGE:3501370 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI147188 cDNA clone IMAGE:5054445 liver 5' read
  AA212768 cDNA clone IMAGE:676838 liver 5' read
  AI391432 cDNA clone IMAGE:676838 liver 5' read Protein similarity
  BF788773 cDNA clone IMAGE:4235419 kidney 5' read
  AW261452 cDNA clone IMAGE:2317925 liver 3' read
  BF782544 cDNA clone IMAGE:4234958 kidney 5' read
  BF780562 cDNA clone IMAGE:4221289 kidney 5' read Protein similarity
  BF137474 cDNA clone IMAGE:4008543 tumor, metastatic to mammary 5' read Protein similarity
  BI692678 cDNA clone IMAGE:5370923 tumor, biopsy sample 5' read Protein similarity
  BI320135 cDNA clone (no-name) 5' read Protein similarity
  AI182190 cDNA clone IMAGE:1430425 mammary gland 5' read Protein similarity
  BE287010 cDNA clone IMAGE:3495971 tumor, gross tissue 5' read Protein similarity
  BF018528 cDNA clone IMAGE:3664122 round spermatids, pooled from multiple mice 5' read Protein similarity
  BE371500 cDNA clone IMAGE:3592129 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  W54489 cDNA clone IMAGE:367889 embryo 5' read Protein similarity
  AA981221 cDNA clone IMAGE:1279465 macrophage 5' read Protein similarity
  BF322101 cDNA clone IMAGE:3812691 liver 5' read Protein similarity
  W88116 cDNA clone IMAGE:407503 embryo 5' read Protein similarity
  BF135684 cDNA clone IMAGE:4008894 tumor, metastatic to mammary 5' read Protein similarity
  BF121915 cDNA clone IMAGE:3992796 tumor, gross tissue 5' read Protein similarity
  BG071728 cDNA clone H3102E06 3' read Protein similarity
  BG084573 cDNA clone H3102E06 5' read Protein similarity
  AU043923 cDNA clone J0912B09 3' read Protein similarity
  AI840129 cDNA clone UI-M-AO0-acc-a-08-0-UI 3' read Protein similarity
  AA734328 cDNA clone IMAGE:1164251 colon 5' read Protein similarity
  AI875712 cDNA clone IMAGE:1972576 kidney 3' read Protein similarity
  AU043925 cDNA clone J0912B11 3' read Protein similarity
  AU043907 cDNA clone J0911H11 3' read Protein similarity
  AA273182 cDNA clone IMAGE:764691 spleen 5' read Protein similarity
  AU044452 cDNA clone J0920A10 3' read Protein similarity
  BE197576 cDNA clone IMAGE:3256965 tumor, gross tissue 3' read
  AA638536 cDNA clone IMAGE:1053713 colon 5' read
  BE634154 cDNA clone IMAGE:3412449 spleen 5' read
  BF319653 cDNA clone IMAGE:3664122 round spermatids, pooled from multiple mice 3' read
  BF322816 cDNA clone IMAGE:3812691 liver 3' read
  AA590577 cDNA clone IMAGE:1025477 colon 5' read
  BI102249 cDNA clone IMAGE:5040831 kidney 5' read
  AA003003 cDNA clone IMAGE:426308 embryo 5' read
  BI148826 cDNA clone IMAGE:5052982 liver 5' read
  BG294368 cDNA clone IMAGE:4503089 retina 5' read