Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.14534
Description:RIKEN cDNA 9130221H12 gene
Best-Of-UniGene (BOU) SequenceAK020285
Genomic Coordiantes Displayed:Bases 232699 to 245712 of contig GA_x5J8B7W585G
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.14534.jpeg
Best sense EST/protein match:No protein match with an e-value of less than 1e-10
Best antisense EST/protein match:BF534846 matched ref|NP_059040.1| (NM_017344) glycogen synthase kinase 3 alpha [Rattus norvegicus] (E = e-100)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI248483 cDNA clone IMAGE:5116574 tumor, biopsy sample 5' read Protein similarity
  BF016587 cDNA clone IMAGE:3661867 tumor, metastatic to mammary 5' read Protein similarity
  AW214809 cDNA clone IMAGE:2650813 tumor, metastatic to mammary 5' read Protein similarity
  BI737187 cDNA clone IMAGE:5365346 retina 5' read Protein similarity
  BF534846 cDNA clone IMAGE:4187540 salivary gland 5' read Protein similarity
  BI217235 cDNA clone IMAGE:5097096 liver 5' read Protein similarity
  BG916343 cDNA clone IMAGE:4937154 tumor, gross tissue 5' read Protein similarity
  BF139115 cDNA clone IMAGE:4011973 tumor, metastatic to mammary 5' read Protein similarity
  BG171931 cDNA clone IMAGE:4456208 tumor, biopsy sample 5' read Protein similarity
  BI655754 cDNA clone IMAGE:5325733 tumor, gross tissue 5' read Protein similarity
  BF584201 cDNA clone IMAGE:4216259 colon 5' read Protein similarity
  AI152926 cDNA clone IMAGE:1479102 uterus 5' read Protein similarity
  BG246603 cDNA clone IMAGE:4488973 tumor, biopsy sample 5' read Protein similarity
  AA681585 cDNA clone IMAGE:1122917 muscle 5' read Protein similarity
  BI646150 cDNA clone IMAGE:5316734 tumor, gross tissue 5' read Protein similarity
  BI684566 cDNA clone IMAGE:5342963 infiltrating ductal carcinoma 5' read Protein similarity
  BI221603 cDNA clone IMAGE:5100410 liver 5' read Protein similarity
  W91360 cDNA clone IMAGE:423863 embryo 5' read Protein similarity
  BE291057 cDNA clone IMAGE:3498066 infiltrating ductal carcinoma 5' read Protein similarity
  BG173923 cDNA clone IMAGE:4457039 tumor, biopsy sample 5' read Protein similarity
  BI738819 cDNA clone IMAGE:5369444 retina 5' read Protein similarity
  BG965064 cDNA clone IMAGE:4983966 colon 5' read Protein similarity
  BI110200 cDNA clone IMAGE:5030619 tumor, gross tissue 5' read Protein similarity
  BG519504 cDNA clone IMAGE:3498066 infiltrating ductal carcinoma 5' read Protein similarity
  AA606844 cDNA clone IMAGE:1005945 embryo 5' read Protein similarity
  BI987522 cDNA clone (no-name) neural retina
  AW213159 cDNA clone IMAGE:2582073 infiltrating ductal carcinoma 5' read Protein similarity
  AA690701 cDNA clone IMAGE:1164087 muscle 5' read Protein similarity
  BG804727 cDNA clone (no-name) neural retina
  BI987440 cDNA clone (no-name) neural retina
  BG261740 cDNA clone IMAGE:4480854 retina 5' read Protein similarity
  AA003230 cDNA clone IMAGE:426918 embryo 5' read Protein similarity
  W65978 cDNA clone IMAGE:386916 embryo 5' read Protein similarity
  AA215076 cDNA clone IMAGE:651527 embryonal carcinoma 5' read Protein similarity
  AA215200 cDNA clone IMAGE:651354 embryonal carcinoma 5' read Protein similarity
  BF016178 cDNA clone IMAGE:3661205 tumor, metastatic to mammary 5' read Protein similarity
  BI144674 cDNA clone IMAGE:5051272 liver 5' read Protein similarity
  BI736478 cDNA clone IMAGE:5367413 retina 5' read Protein similarity
  W53296 cDNA clone IMAGE:370924 embryo 5' read Protein similarity
  W57139 cDNA clone IMAGE:372056 embryo 5' read Protein similarity
  BE381082 cDNA clone IMAGE:3600894 tumor, biopsy sample 5' read Protein similarity
  W70619 cDNA clone IMAGE:390276 embryo 5' read Protein similarity
  AA474346 cDNA clone IMAGE:804504 embryo 5' read Protein similarity
  AA269836 cDNA clone IMAGE:735743 embryo 5' read Protein similarity
  AA980094 cDNA clone IMAGE:1348064 mammary gland 5' read Protein similarity
  AI427293 cDNA clone IMAGE:355707 embryo 5' read
  W47728 cDNA clone IMAGE:355707 embryo 5' read
  BE914094 cDNA clone IMAGE:3965379 tumor, biopsy sample 5' read
  BE373269 cDNA clone IMAGE:3583563 tumor, biopsy sample 5' read
  AA034715 cDNA clone IMAGE:467479 embryo 5' read
  AA048905 cDNA clone IMAGE:479226 embryo 5' read
  AA221890 cDNA clone IMAGE:658669 embryo 5' read
  AA968126 cDNA clone IMAGE:1617655 hypothalamus 5' read
  W64641 cDNA clone IMAGE:373230 embryo 5' read
  BF531637 cDNA clone IMAGE:4205522 colon 5' read Protein similarity
  BI683765 cDNA clone IMAGE:5342289 infiltrating ductal carcinoma 5' read
  BG864594 cDNA clone IMAGE:4919555 tumor, gross tissue 5' read
  AA030429 cDNA clone IMAGE:464446 embryo 5' read
  AA611176 cDNA clone IMAGE:1054520 mammary gland 5' read
  AA221558 cDNA clone IMAGE:670504 embryo 5' read
  AI465268 cDNA clone IMAGE:658669 embryo 3' read
  AI413327 cDNA clone IMAGE:355707 embryo 3' read Polyadenylation signal
  BE335197 cDNA clone IMAGE:2650813 tumor, metastatic to mammary 3' read Polyadenylation signal
  BG808297 cDNA clone (no-name) neural retina Polyadenylation signal
  AI893458 cDNA clone IMAGE:616541 embryonal carcinoma 3' read Polyadenylation signal

SENSE ESTs

  BG070719 cDNA clone H3090D07 3' read
  AU016786 cDNA clone J0729G02 embryo 3' read Protein similarity
  AI642704 cDNA clone IMAGE:1230916 mammary gland 3' read
  AI642705 cDNA clone IMAGE:1230928 mammary gland 3' read
  AA790414 cDNA clone IMAGE:1230928 mammary gland 5' read Protein similarity
  BF585417 cDNA clone IMAGE:4207058 colon 5' read
  BF099810 cDNA clone IMAGE:3979140 tumor, biopsy sample 5' read
  BI646781 cDNA clone IMAGE:5316820 tumor, gross tissue 5' read
  BG864253 cDNA clone IMAGE:4918513 tumor, gross tissue 5' read