Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.150686
Description:RIKEN cDNA 1110013J05 gene
Best-Of-UniGene (BOU) SequenceAY065980
Genomic Coordiantes Displayed:Bases 486842 to 588570 of contig GA_x5J8B7W44N3
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.150686.jpeg
Best sense EST/protein match:AY065980 matched gb|AAL47579.1| (AY065980) helicase DDX32 [Mus musculus] (E = 0.0)
Best antisense EST/protein match:BE915154 matched dbj|BAB27116.1| (AK010684) data source:SPTR, source key:Q9P287, (E = e-110)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AI482545 cDNA clone IMAGE:864810 mammary gland 3' read
  AA444820 cDNA clone IMAGE:864810 mammary gland 5' read Protein similarity
  AI614722 cDNA clone IMAGE:864810 mammary gland 5' read Protein similarity
  BF454041 cDNA clone IMAGE:3810347 salivary gland 5' read
  BG075711 cDNA clone H3150F12 3' read Polyadenylation signal
  AW556933 cDNA clone L0275B10 3' read Protein similarityPolyadenylation signal
  AI255328 cDNA clone IMAGE:1889868 liver 3' read Polyadenylation signal
  AA230894 cDNA clone IMAGE:670714 embryo 5' read
  AI427589 cDNA clone IMAGE:522084 muscle 3' read Polyadenylation signal
  BI081107 cDNA clone IMAGE:5010646 tumor, biopsy sample 5' read
  AA008959 cDNA clone IMAGE:440990 embryo 5' read
  BG870752 cDNA clone IMAGE:4923235 salivary gland 5' read
  AA209602 cDNA clone IMAGE:676549 liver 5' read
  W85130 cDNA clone IMAGE:408145 embryo 5' read
  BG914658 cDNA clone IMAGE:4935700 tumor, gross tissue 5' read Protein similarity
  BE915154 cDNA clone IMAGE:3966386 tumor, biopsy sample 5' read Protein similarity
  AA592017 cDNA clone IMAGE:947250 embryo 5' read
  BG088254 cDNA clone H3150F12 5' read Protein similarity
  AI020725 cDNA clone IMAGE:1365565 mammary gland 5' read
  BI789583 cDNA clone (no-name) 5' read
  BE290366 cDNA clone IMAGE:3483353 tumor, gross tissue 5' read Protein similarity
  AI552770 cDNA clone IMAGE:522084 muscle 5' read
  AI530373 cDNA clone IMAGE:1889868 liver 5' read Protein similarity
  C89261 cDNA clone 01B00061JE01 embryo Protein similarity
  AA106130 cDNA clone IMAGE:522084 muscle 5' read

SENSE ESTs

  AA003735 cDNA clone IMAGE:437266 embryo 5' read Polyadenylation signal
  AI156803 cDNA clone IMAGE:1885234 embryo 5' read
  BF179649 cDNA clone IMAGE:4037168 tumor, gross tissue 5' read Protein similarity
  AI788191 cDNA clone IMAGE:2087929 embryo 5' read
  AA104201 cDNA clone IMAGE:556941 embryo 5' read
  BG172858 cDNA clone IMAGE:4460064 tumor, biopsy sample 5' read Protein similarity
  BE381007 cDNA clone IMAGE:3600851 tumor, biopsy sample 5' read Protein similarity
  AA408141 cDNA clone C0023G08 embryo 5' read Protein similarity
  AA716835 cDNA clone IMAGE:1195962 mammary gland 5' read Protein similarity
  BF302905 cDNA clone IMAGE:4167622 salivary gland 5' read Protein similarity
  BG963436 cDNA clone IMAGE:4982468 colon 5' read Protein similarity
  AA794812 cDNA clone IMAGE:1196180 mammary gland 5' read Protein similarity
  AA048310 cDNA clone IMAGE:477395 embryo 5' read Protein similarity
  W97211 cDNA clone IMAGE:423172 embryo 5' read Protein similarity
  BG967556 cDNA clone IMAGE:4987737 colon 5' read Protein similarity
  BI986455 cDNA clone (no-name) neural retina
  BE375911 cDNA clone IMAGE:3593361 tumor, biopsy sample 5' read Protein similarity
  BF098887 cDNA clone IMAGE:3978688 tumor, biopsy sample 5' read Protein similarity
  AI007263 cDNA clone IMAGE:1348572 mammary gland 5' read Protein similarity
  BF151342 cDNA clone IMAGE:3669116 tumor, gross tissue 5' read Protein similarity
  BG695677 cDNA clone IMAGE:4318143 pituitary gland 5' read Protein similarity
  BI696291 cDNA clone IMAGE:5373497 tumor, biopsy sample 5' read Protein similarity
  BI154769 cDNA clone IMAGE:5034138 tumor, gross tissue 5' read Protein similarity
  AA170577 cDNA clone IMAGE:617830 spleen 5' read Protein similarity
  AW823312 cDNA clone IMAGE:3155749 tumor, gross tissue 5' read Protein similarity
  AW764137 cDNA clone IMAGE:3155749 tumor, gross tissue 3' read Protein similarity
  BE199108 cDNA clone IMAGE:3164316 tumor, biopsy sample 3' read
  AA529994 cDNA clone IMAGE:921748 colon 5' read
  BE849835 cDNA clone IMAGE:3416076 spleen 5' read Protein similarity
  AA008692 cDNA clone IMAGE:439619 embryo 5' read
  AA914379 cDNA clone IMAGE:1313877 mammary gland 5' read
  AU017568 cDNA clone J0740H09 embryo 3' read
  AI117650 cDNA clone IMAGE:1885234 embryo 3' read Polyadenylation signal
  AU043228 cDNA clone J0901G12 3' read Polyadenylation signal
  AA220766 cDNA clone IMAGE:660068 embryo 5' read Protein similarityPolyadenylation signal
  BI439346 cDNA clone (no-name) 5' read
  BI499601 cDNA clone (no-name) 5' read
  AI643891 cDNA clone IMAGE:1195962 mammary gland 3' read Polyadenylation signal
  AA000713 cDNA clone IMAGE:425876 embryo 5' read
  AI882336 cDNA clone IMAGE:1396600 mammary gland 5' read
  BI220335 cDNA clone IMAGE:5098710 liver 5' read
  AA880270 cDNA clone IMAGE:1230590 mammary gland 5' read
  AA981920 cDNA clone IMAGE:1348619 mammary gland 5' read
  W97510 cDNA clone IMAGE:422264 embryo 5' read
  W59178 cDNA clone IMAGE:374233 embryo 5' read
  AA014608 cDNA clone IMAGE:468506 embryo 5' read
  W65675 cDNA clone IMAGE:387403 embryo 5' read
  AA049597 cDNA clone IMAGE:478145 embryo 5' read
  AI882469 cDNA clone IMAGE:1396804 mammary gland 5' read
  AA268827 cDNA clone IMAGE:747688 liver 5' read