Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.178115
Description:RIKEN cDNA 1300010K09 gene
Best-Of-UniGene (BOU) SequenceBC003289
Genomic Coordiantes Displayed:Bases 11342528 to 11348735 of contig GA_x5J8B7W6RL5
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.178115.jpeg
Best sense EST/protein match:BC003289 matched gb|AAH03289.1|AAH03289 (BC003289) Similar to hypothetical protein MGC3262 [Mus musculus] (E = e-163)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BF580120 cDNA clone IMAGE:4219087 colon 5' read Polyadenylation signal
  AA162220 cDNA clone IMAGE:599038 spleen 5' read
  BE199159 cDNA clone IMAGE:3164359 tumor, biopsy sample 3' read Polyadenylation signal
  BF148817 cDNA clone IMAGE:3661535 tumor, metastatic to mammary 3' read Polyadenylation signal
  AW414050 cDNA clone IMAGE:2650623 tumor, gross tissue 3' read Polyadenylation signal
  BF449982 cDNA clone IMAGE:3814015 brain 3' read Polyadenylation signal
  BF449540 cDNA clone IMAGE:3814015 brain 5' read
  W54746 cDNA clone IMAGE:367975 embryo 5' read
  AW538664 cDNA clone C0110D01 3' read Polyadenylation signal
  BF321245 cDNA clone IMAGE:3673478 infiltrating ductal carcinoma 3' read Polyadenylation signal
  W75178 cDNA clone IMAGE:390593 embryo 5' read
  AA271789 cDNA clone IMAGE:736900 embryo 5' read
  BE534232 cDNA clone IMAGE:3595960 infiltrating ductal carcinoma 5' read
  AA268098 cDNA clone IMAGE:746708 embryo 5' read
  BE292679 cDNA clone IMAGE:3158000 infiltrating ductal carcinoma 3' read
  AA510628 cDNA clone IMAGE:863522 mammary gland 5' read
  AA272279 cDNA clone IMAGE:761526 pool 5' read
  AA259848 cDNA clone IMAGE:733494 embryo 5' read
  AA980545 cDNA clone IMAGE:1349418 mammary gland 5' read
  AA161968 cDNA clone IMAGE:540365 embryo 5' read
  AA123173 cDNA clone IMAGE:538850 embryo 5' read
  W64905 cDNA clone IMAGE:386830 embryo 5' read
  W70711 cDNA clone IMAGE:390198 embryo 5' read
  AA472517 cDNA clone IMAGE:874253 mammary gland 5' read

SENSE ESTs

  AA967152 cDNA clone IMAGE:1349245 mammary gland 5' read Protein similarity
  AA537125 cDNA clone IMAGE:949527 mammary gland 5' read Protein similarity
  BI219486 cDNA clone IMAGE:5100084 liver 5' read Protein similarity
  AA981874 cDNA clone IMAGE:1348857 mammary gland 5' read Protein similarity
  AW319050 cDNA clone IMAGE:2332293 kidney 5' read
  BG868066 cDNA clone IMAGE:4913341 salivary gland 5' read Protein similarity
  BE372661 cDNA clone IMAGE:3583087 tumor, biopsy sample 5' read Protein similarity
  BE554252 cDNA clone IMAGE:3152711 tumor, biopsy sample 5' read Protein similarity
  BF166793 cDNA clone IMAGE:3995666 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI649171 cDNA clone IMAGE:5319163 tumor, gross tissue 5' read Protein similarity
  BI663420 cDNA clone IMAGE:5321397 infiltrating ductal carcinoma 5' read Protein similarity
  BF302244 cDNA clone IMAGE:4166568 salivary gland 5' read Protein similarity
  BE305854 cDNA clone IMAGE:3495549 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  AW261740 cDNA clone IMAGE:2332293 kidney 3' read
  AW215027 cDNA clone IMAGE:2651000 tumor, metastatic to mammary 5' read Protein similarity
  BF235533 cDNA clone IMAGE:4160581 liver 5' read
  AW907962 cDNA clone IMAGE:3158000 infiltrating ductal carcinoma 5' read
  BE311185 cDNA clone IMAGE:3158000 infiltrating ductal carcinoma 5' read
  BE376609 cDNA clone IMAGE:3584841 tumor, biopsy sample 5' read
  AI987990 cDNA clone IMAGE:2182635 kidney 3' read
  AI647525 cDNA clone IMAGE:1971512 kidney 3' read
  AI644537 cDNA clone IMAGE:554594 embryo 3' read Polyadenylation signal