Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.196480
Description:DNA segment, Human EST J0827E04
Best-Of-UniGene (BOU) SequenceAK006539
Genomic Coordiantes Displayed:Bases 1375634 to 1385192 of contig GA_x5J8B7W53WT
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.196480.jpeg
Best sense EST/protein match:AK006539 matched dbj|BAB24642.1| (AK006539) DNA segment, Human EST J0827E04~data source:MGD, source (E = e-119)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10

This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

  BI251826 cDNA clone IMAGE:5151704 tumor, gross tissue 5' read
  BG920916 cDNA clone IMAGE:4951615 infiltrating ductal carcinoma 5' read
  BI154837 cDNA clone IMAGE:5032324 tumor, gross tissue 5' read
  BF788363 cDNA clone IMAGE:4242288 kidney 5' read
  BF158919 cDNA clone IMAGE:3982293 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read


  BG071426 cDNA clone H3098G12 3' read Polyadenylation signal
  AU041213 cDNA clone J0827E04 3' read Polyadenylation signal
  BG084295 cDNA clone H3098G12 5' read
  AA008475 cDNA clone IMAGE:439777 embryo 5' read
  AI466666 cDNA clone IMAGE:439777 embryo 5' read