Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.196749
Description:RIKEN cDNA 2610100B20 gene
Best-Of-UniGene (BOU) SequenceBC016616
Genomic Coordiantes Displayed:Bases 1834057 to 1908150 of contig GA_x5J8B7W38CT
BOU Orientation Along Contig:RIGHT-TO-LEFT with respect to contig
Link to JPEG of genomic mappingMm.196749.jpeg
Best sense EST/protein match:AI425694 matched gb|AAH16616.1|AAH16616 (BC016616) Unknown (protein for MGC:11904) [Mus musculus] (E = e-101)
Best antisense EST/protein match:AW553211 matched gb|AAH08103.1|AAH08103 (BC008103) Similar to hypothetical protein FLJ10504 [Mus musculus] (E = e-107)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI103868 cDNA clone IMAGE:5035560 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI099869 cDNA clone IMAGE:5039997 kidney 5' read Protein similarity
  BG078571 cDNA clone H3029C12 5' read Protein similarity
  BF301580 cDNA clone IMAGE:4164830 salivary gland 5' read
  BE916171 cDNA clone IMAGE:3966815 tumor, biopsy sample 5' read Protein similarity
  BF160492 cDNA clone IMAGE:3988070 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI696985 cDNA clone IMAGE:5376081 tumor, biopsy sample 5' read Protein similarity
  BE367985 cDNA clone IMAGE:3591014 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI900426 cDNA clone (no-name) 5' read Protein similarity
  BI900425 cDNA clone (no-name) 5' read Protein similarity
  BG176510 cDNA clone IMAGE:4457876 tumor, biopsy sample 5' read Protein similarity
  AL118127 cDNA clone 528_10I13 dissected endoderm 5' read Protein similarity
  BI105882 cDNA clone IMAGE:5037388 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  AA276079 cDNA clone IMAGE:775875 kidney 5' read
  W88052 cDNA clone IMAGE:419154 embryo 5' read
  AA498195 cDNA clone IMAGE:889244 pool 5' read
  AA498416 cDNA clone IMAGE:889767 pool 5' read
  AW539725 cDNA clone C0123F09 3' read Protein similarityPolyadenylation signal
  AI037633 cDNA clone IMAGE:1746353 testis 5' read
  AW553211 cDNA clone L0223F05 3' read Protein similarityPolyadenylation signal
  AW537169 cDNA clone G0113B08 3' read Protein similarityPolyadenylation signal
  BG065284 cDNA clone H3029C12 3' read Polyadenylation signal
  BE633056 cDNA clone IMAGE:3412726 spleen 5' read
  BE685992 cDNA clone IMAGE:3412726 spleen 3' read
  AA591266 cDNA clone IMAGE:990891 embryo 5' read
  BI900114 cDNA clone (no-name) 3' read Polyadenylation signal
  BI900115 cDNA clone (no-name) 3' read Polyadenylation signal
  AA003100 cDNA clone IMAGE:427220 embryo 5' read
  BG805499 cDNA clone (no-name) neural retina

SENSE ESTs

  BF226284 cDNA clone IMAGE:3672424 infiltrating ductal carcinoma 3' read
  W80163 cDNA clone IMAGE:402512 embryo 5' read
  BF225978 cDNA clone IMAGE:3673275 infiltrating ductal carcinoma 3' read
  AW215633 cDNA clone IMAGE:2651328 tumor, metastatic to mammary 3' read
  AW413320 cDNA clone IMAGE:2631172 infiltrating ductal carcinoma 3' read Protein similarity
  AI413931 cDNA clone IMAGE:400287 embryo 3' read
  BG066100 cDNA clone H3038H08 3' read
  C78031 cDNA clone J0041G03 embryo 3' read Polyadenylation signal
  BE377807 cDNA clone IMAGE:3594087 tumor, biopsy sample 5' read
  BI104453 cDNA clone IMAGE:5034972 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF162133 cDNA clone IMAGE:3989809 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF161302 cDNA clone IMAGE:3989558 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BG079365 cDNA clone H3038H08 5' read
  BI148349 cDNA clone IMAGE:5055496 liver 5' read
  AI425694 cDNA clone IMAGE:400287 embryo 5' read
  W77564 cDNA clone IMAGE:400287 embryo 5' read
  BG803820 cDNA clone (no-name) neural retina
  BE573158 cDNA clone IMAGE:3709155 infiltrating ductal carcinoma 5' read
  BG973925 cDNA clone IMAGE:4979462 tumor, gross tissue 5' read
  AI007287 cDNA clone IMAGE:1364061 mammary gland 5' read
  BI106255 cDNA clone IMAGE:5035938 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF015773 cDNA clone IMAGE:3660924 tumor, metastatic to mammary 5' read
  AL363201 cDNA clone R9222B18 embryo 5' read
  BI735114 cDNA clone IMAGE:5363484 retina 5' read
  AW215205 cDNA clone IMAGE:2651328 tumor, metastatic to mammary 5' read
  BE535161 cDNA clone IMAGE:3598688 infiltrating ductal carcinoma 5' read
  BG808125 cDNA clone (no-name) neural retina
  BE912914 cDNA clone IMAGE:3965250 tumor, biopsy sample 5' read
  BI556667 cDNA clone IMAGE:5292282 tumor, gross tissue 5' read
  BF226515 cDNA clone IMAGE:3672424 infiltrating ductal carcinoma 5' read