Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.19726
Description:dynein, axon, heavy chain 11
Best-Of-UniGene (BOU) SequenceNM_010060
Genomic Coordiantes Displayed:Bases 1668253 to 2148562 of contig GA_x5J8B7W4MS8
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.19726.jpeg
Best sense EST/protein match:BF538185 matched ref|NP_034190.1| (NM_010060) dynein, axon, heavy chain 11; situs inversus viscerum (E = e-119)
Best antisense EST/protein match:BI654001 matched gb|AAH06933.1|AAH06933 (BC006933) Unknown (protein for MGC:11998) [Mus musculus] (E = e-102)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BI153005 cDNA clone IMAGE:5068535 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  AI326265 cDNA clone IMAGE:474743 embryo 3' read Protein similarityPolyadenylation signal
  BF319124 cDNA clone IMAGE:3663217 tumor, metastatic to mammary 3' read Protein similarityPolyadenylation signal
  BF017422 cDNA clone IMAGE:3654120 type b spermatogonia, pooled from multiple mice 3' read Protein similarityPolyadenylation signal
  AI314784 cDNA clone IMAGE:1921934 kidney 3' read Protein similarityPolyadenylation signal
  BE336209 cDNA clone IMAGE:3325278 spleen 5' read Protein similarity
  AI322623 cDNA clone IMAGE:474743 embryo 5' read
  BI527015 cDNA clone IMAGE:5059533 tumor, biopsy sample 5' read Protein similarity
  AU016064 cDNA clone J0720B12 embryo 3' read Protein similarity
  BG070465 cDNA clone H3087F06 3' read Protein similarity
  AA039110 cDNA clone IMAGE:474743 embryo 5' read Protein similarity
  BF012746 cDNA clone IMAGE:3654120 type b spermatogonia, pooled from multiple mice 5' read Protein similarity