Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.1994
Description:TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80 kDa
Best-Of-UniGene (BOU) SequenceNM_009315
Genomic Coordiantes Displayed:Bases 82834 to 95124 of contig GA_x5J8B7W3K0W
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.1994.jpeg
Best sense EST/protein match:BI647468 matched ref|NP_033341.1| (NM_009315) TAF6 RNA polymerase II, TATA box binding protein (E = e-127)
Best antisense EST/protein match:No protein match with an e-value of less than 1e-10


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AW214268 cDNA clone IMAGE:2645518 spontaneous tumor, metastatic to mammary. stem cell origin. 3' read
  AW555116 cDNA clone L0250B10 3' read Polyadenylation signal
  BF135891 cDNA clone IMAGE:4009324 tumor, metastatic to mammary 5' read Protein similarity
  BI664698 cDNA clone IMAGE:5322331 infiltrating ductal carcinoma 5' read Protein similarity
  AW229978 cDNA clone IMAGE:2645518 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BF160897 cDNA clone IMAGE:3988347 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BE367935 cDNA clone IMAGE:3590808 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BE371631 cDNA clone IMAGE:3592094 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity

SENSE ESTs

  BF535015 cDNA clone IMAGE:4189864 salivary gland 5' read Protein similarity
  BI657604 cDNA clone IMAGE:5325712 tumor, gross tissue 5' read Protein similarity
  BG863114 cDNA clone IMAGE:4918963 tumor, gross tissue 5' read Protein similarity
  BI147344 cDNA clone IMAGE:5054611 liver 5' read Protein similarity
  BI647468 cDNA clone IMAGE:5318769 tumor, gross tissue 5' read Protein similarity
  AW214759 cDNA clone IMAGE:2650798 tumor, metastatic to mammary 5' read Protein similarity
  BI652115 cDNA clone IMAGE:5339524 tumor, gross tissue 5' read Protein similarity
  BI149754 cDNA clone IMAGE:5011974 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read Protein similarity
  BI687324 cDNA clone IMAGE:5353302 infiltrating ductal carcinoma 5' read Protein similarity
  BE912338 cDNA clone IMAGE:3965049 tumor, biopsy sample 5' read Protein similarity
  AI893946 cDNA clone IMAGE:389161 embryo 5' read Protein similarity
  W74881 cDNA clone IMAGE:389161 embryo 5' read Protein similarity
  BI079367 cDNA clone IMAGE:5005499 tumor, biopsy sample 5' read Protein similarity
  BI687880 cDNA clone IMAGE:5354236 infiltrating ductal carcinoma 5' read Protein similarityPolyadenylation signal
  BF152974 cDNA clone IMAGE:3809742 testis 5' read Polyadenylation signal