Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.200507
Description:tubulin-specific chaperone e
Best-Of-UniGene (BOU) SequenceAK011899
Genomic Coordiantes Displayed:Bases 3429152 to 3549083 of contig GA_x5J8B7W6JPT
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.200507.jpeg
Best sense EST/protein match:AK011899 matched ref|NP_003184.1| (NM_003193) beta-tubulin cofactor E [Homo sapiens] (E = 1e-94)
Best antisense EST/protein match:BF165476 matched gb|AAH16974.1|AAH16974 (BC016974) Unknown (protein for IMAGE:4393885) [Homo sapiens] (E = 9e-81)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  AA921054 cDNA clone IMAGE:1312856 macrophage 5' read Protein similarity
  BG969326 cDNA clone IMAGE:4971241 kidney 5' read
  BE288907 cDNA clone IMAGE:3488991 tumor, gross tissue 5' read
  AA427154 cDNA clone IMAGE:832405 mammary gland 5' read
  BI453317 cDNA clone IMAGE:5252313 tumor, gross tissue 5' read
  AW762616 cDNA clone IMAGE:3155064 tumor, gross tissue 5' read
  AA833054 cDNA clone IMAGE:1433240 uterus 5' read
  AA000448 cDNA clone IMAGE:401543 embryo 5' read
  BE281788 cDNA clone IMAGE:3492207 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AU043040 cDNA clone J1037H05 3' read
  BG072521 cDNA clone H3111G05 3' read
  BG923691 cDNA clone IMAGE:4950189 infiltrating ductal carcinoma 5' read
  BI985521 cDNA clone (no-name) neural retina
  BG085316 cDNA clone H3111G05 5' read
  AA166478 cDNA clone IMAGE:614634 embryo 5' read
  BF165476 cDNA clone IMAGE:4019033 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BI665080 cDNA clone IMAGE:5321438 infiltrating ductal carcinoma 5' read
  BE305298 cDNA clone IMAGE:3492242 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  BF162091 cDNA clone IMAGE:3987756 spontaneous tumor, metastatic to mammary. stem cell origin. 5' read
  AW611066 cDNA clone IMAGE:2536822 kidney 5' read
  BB610080 cDNA clone 1300008J18 liver 5' read
  BI102749 cDNA clone IMAGE:5043415 kidney 5' read

SENSE ESTs

  BG342259 cDNA clone IMAGE:4481770 retina 5' read Protein similarity
  BG245277 cDNA clone IMAGE:4486031 tumor, biopsy sample 5' read Protein similarity
  BI794006 cDNA clone (no-name) pancreas 5' read Protein similarity
  BI990635 cDNA clone (no-name) neural retina
  BG862096 cDNA clone IMAGE:4916673 tumor, gross tissue 5' read Protein similarity
  AW911224 cDNA clone IMAGE:3156911 infiltrating ductal carcinoma 5' read
  AA175351 cDNA clone IMAGE:618182 spleen 5' read Protein similarity
  AA050111 cDNA clone IMAGE:476606 embryo 5' read Protein similarity
  BG799985 cDNA clone (no-name) neural retina
  AW558517 cDNA clone L0295A05 3' read Protein similarityPolyadenylation signal
  AW229329 cDNA clone IMAGE:2599024 infiltrating ductal carcinoma 5' read
  BI793703 cDNA clone (no-name) pancreas 3' read Polyadenylation signal
  AI664187 cDNA clone IMAGE:1497652 uterus 5' read Protein similarity
  AW823239 cDNA clone IMAGE:3156911 infiltrating ductal carcinoma 3' read Protein similarityPolyadenylation signal
  AW212599 cDNA clone IMAGE:2599024 infiltrating ductal carcinoma 3' read Protein similarityPolyadenylation signal