Computational Discovery of Overlapping Transcriptional Units in the Human & Mouse Genomes

Candidate UniGene cluster:UniGene Cluster Mm.210047
Description:RIKEN cDNA 2010309H15 gene
Best-Of-UniGene (BOU) SequenceAF416923
Genomic Coordiantes Displayed:Bases 5974065 to 6047288 of contig GA_x5J8B7W55JN
BOU Orientation Along Contig:LEFT-TO-RIGHT with respect to contig
Link to JPEG of genomic mappingMm.210047.jpeg
Best sense EST/protein match:AF416923 matched gb|AAL40939.1|AF416923_1 (AF416923) brown fat inducible thioesterase 2 [Mus musculus] (E = 0.0)
Best antisense EST/protein match:AI314014 matched gb|AAH20874.1|AAH20874 (BC020874) Unknown (protein for MGC:24088) [Homo sapiens] (E = 5e-73)


This page provides information on exon-intron structure, mouse-human homologies, polyA signals & locations, and protein homologies of ESTs from directionally-cloned libraries that are members of a UniGene cluster that is a candidate for containing ESTs derived from distinct, overlapping sense & antisense transcripts.
The upper portion of the graph is reserved for the exon-intron mappings of ESTs oriented consistently with the Best-Of-UniGene (BOU) sequence (in gold). The lower portion of the graph is reserved for the exon-intron mappings of ESTs oriented opposite the BOU sequence (in white). Dividing these portions are the exon-intron mapping of the BOU sequence itself (in blue and green). Immedietely below the BOU are HUMMUS-defined streches of sequences highly conserved between mouse and human. The height of each bar is reflective of the % identity of human-mouse homology over a 100 base-pair window centered at a given base. At the left of the graph, the GenBank accession number of the BOU or EST sequence represented along that Y-axis position is listed, along with the UniLib identification number of the library from which that EST was derived.Below the graph, the GenBank accession numbers of each of the sense & antisense ESTs are provided, along with information on which have polyA signals and protein homologies, as determined by the NCBI.

Below are listed the same set of ESTs as is depicted above, with information of read direction, tissue type, protein similarity (P), and poly-adenylation signal (A).

ANTISENSE ESTs
  BG970975 cDNA clone IMAGE:4974570 kidney 5' read
  AI481864 cDNA clone IMAGE:934865 embryo 3' read Polyadenylation signal
  BG066548 cDNA clone H3045C01 3' read Polyadenylation signal
  AI956935 cDNA clone IMAGE:2135957 kidney 3' read Polyadenylation signal
  AU043429 cDNA clone J0904G08 3' read Polyadenylation signal
  C80245 cDNA clone J0078F04 embryo 3' read Polyadenylation signal
  C79821 cDNA clone J0072D12 embryo 3' read Polyadenylation signal
  C80588 cDNA clone J0084F04 embryo 3' read Polyadenylation signal
  AI875650 cDNA clone IMAGE:1972498 kidney 3' read Polyadenylation signal
  AI957339 cDNA clone IMAGE:2158938 kidney 3' read
  AI790560 cDNA clone IMAGE:2064909 kidney 3' read
  AI874959 cDNA clone IMAGE:2099566 kidney 3' read
  AI648930 cDNA clone IMAGE:1970766 kidney 3' read
  AI788530 cDNA clone IMAGE:1972013 kidney 3' read
  AI314014 cDNA clone IMAGE:1920902 kidney 3' read
  AA286114 cDNA clone IMAGE:776415 kidney 5' read
  BI143766 cDNA clone IMAGE:5064271 kidney 5' read
  AI508837 cDNA clone IMAGE:776415 kidney 5' read

SENSE ESTs

  W64130 cDNA clone IMAGE:385649 embryo 5' read Protein similarity
  AA051663 cDNA clone IMAGE:479707 embryo 5' read Protein similarity
  BF780247 cDNA clone IMAGE:4221466 kidney 5' read Protein similarity
  BF786458 cDNA clone IMAGE:4240966 kidney 5' read Protein similarity
  BF134551 cDNA clone IMAGE:4012743 tumor, metastatic to mammary 5' read Protein similarity
  AA036034 cDNA clone IMAGE:469019 embryo 5' read Protein similarity
  BI100961 cDNA clone IMAGE:5041677 kidney 5' read Protein similarity